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Newcastle Disease Virus V Protein Inhibits Cell Apoptosis and Promotes Viral Replication by Targeting CacyBP/SIP.
Chu, Zhili; Wang, Caiying; Tang, Qiuxia; Shi, Xiaolei; Gao, Xiaolong; Ma, Jiangang; Lu, Kejia; Han, Qingsong; Jia, Yanqing; Wang, Xiangwei; Adam, Fathalrhman Eisa Addoma; Liu, Haijin; Xiao, Sa; Wang, Xinglong; Yang, Zengqi.
Afiliación
  • Chu Z; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
  • Wang C; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
  • Tang Q; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
  • Shi X; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
  • Gao X; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
  • Ma J; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
  • Lu K; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
  • Han Q; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
  • Jia Y; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
  • Wang X; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
  • Adam FEA; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
  • Liu H; Department of Preventive Medicine and Public Health, Faculty of Veterinary Science, University of Nyala, Nyala, Sudan.
  • Xiao S; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
  • Wang X; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
  • Yang Z; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
Front Cell Infect Microbiol ; 8: 304, 2018.
Article en En | MEDLINE | ID: mdl-30234028
Newcastle disease virus (NDV) has been classified by the World Organization for Animal Health (OIE) as a notable disease-causing virus, and this virus has the ability to infect a wide range of birds. V protein is a non-structural protein of NDV. V protein has been reported to inhibit cell apoptosis (Park et al., 2003a) and promote viral replication (Huang et al., 2003), however, the mechanisms of action of V protein have not been elucidated. In the present study, a yeast two-hybrid screen was performed, and V protein was found to interact with the CacyBP/SIP protein. The results of co-immunoprecipitation and immuno-colocalization assays confirmed the interaction between V protein and CacyBP/SIP. The results of quantitative-PCR and viral plaque assays showed that overexpression of CacyBP/SIP inhibited viral replication in DF-1 cells. Overexpression of CacyBP/SIP in DF-1 cells induced caspase3-dependent apoptosis. The effect of knocking down CacyBP/SIP by siRNA was the opposite of that observed upon overexpression. Moreover, it is known that NDV induces cell apoptosis via multiple caspase-dependent pathways. Furthermore, V protein inhibited cell apoptosis and downregulated CacyBP/SIP expression in DF-1 cells. Taken together, the findings of the current study indicate that V protein interacts with CacyBP/SIP, thereby regulating cell apoptosis and viral replication.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Virales / Replicación Viral / Virus de la Enfermedad de Newcastle / Proteínas de Unión al Calcio / Apoptosis / Proteínas Adaptadoras Transductoras de Señales / Interacciones Huésped-Patógeno Límite: Animals / Humans Idioma: En Revista: Front Cell Infect Microbiol Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Virales / Replicación Viral / Virus de la Enfermedad de Newcastle / Proteínas de Unión al Calcio / Apoptosis / Proteínas Adaptadoras Transductoras de Señales / Interacciones Huésped-Patógeno Límite: Animals / Humans Idioma: En Revista: Front Cell Infect Microbiol Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza