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1H NMR Metabolomics Identifies Underlying Inflammatory Pathology in Osteoarthritis and Rheumatoid Arthritis Synovial Joints.
Anderson, James R; Chokesuwattanaskul, Susama; Phelan, Marie M; Welting, Tim J M; Lian, Lu-Yun; Peffers, Mandy J; Wright, Helen L.
Afiliación
  • Anderson JR; Institute of Ageing and Chronic Disease , University of Liverpool , Liverpool L7 8TX , U.K.
  • Chokesuwattanaskul S; Institute of Integrative Biology , University of Liverpool , Liverpool L69 7ZB , U.K.
  • Phelan MM; Chulalongkorn University , Bangkok 10330 , Thailand.
  • Welting TJM; Institute of Integrative Biology , University of Liverpool , Liverpool L69 7ZB , U.K.
  • Lian LY; HLS Technology Directorate , University of Liverpool , Liverpool L7 8TX , U.K.
  • Peffers MJ; Laboratory for Experimental Orthopedics, Department of Orthopedic Surgery , Maastricht University Medical Centre , 6229 HX Maastricht , The Netherlands.
  • Wright HL; Institute of Integrative Biology , University of Liverpool , Liverpool L69 7ZB , U.K.
J Proteome Res ; 17(11): 3780-3790, 2018 11 02.
Article en En | MEDLINE | ID: mdl-30229649
Despite osteoarthritis (OA) and rheumatoid arthritis (RA) being typically age-related, their underlying etiologies are markedly different. We used 1H nuclear magnetic resonance (NMR) spectroscopy to identify differences in metabolite profiles in low volumes of OA and RA synovial fluid (SF). SF was aspirated from knee joints of 10 OA and 14 RA patients. 100 µL SF was analyzed using a 700 MHz Avance IIIHD Bruker NMR spectrometer with a TCI cryoprobe. Spectra were analyzed by Chenomx, Bruker TopSpin and AMIX software. Statistical analysis was undertaken using Metaboanalyst. 50 metabolites were annotated, including amino acids, saccharides, nucleotides and soluble lipids. Discriminant analysis identified group separation between OA and RA cohorts, with 32 metabolites significantly different between OA and RA SF (false discovery rate (FDR) < 0.05). Metabolites of glycolysis and the tricarboxylic acid cycle were lower in RA compared to OA; these results concur with higher levels of inflammation, synovial proliferation and hypoxia found in RA compared to OA. Elevated taurine in OA may indicate increased subchondral bone sclerosis. We demonstrate that quantifiable differences in metabolite abundance can be measured in low volumes of SF by 1H NMR spectroscopy, which may be clinically useful to aid diagnosis and improve understanding of disease pathogenesis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoartritis / Artritis Reumatoide / Líquido Sinovial / Espectroscopía de Resonancia Magnética / Metaboloma / Metabolómica Tipo de estudio: Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoartritis / Artritis Reumatoide / Líquido Sinovial / Espectroscopía de Resonancia Magnética / Metaboloma / Metabolómica Tipo de estudio: Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos