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Changes in neuromuscular structure and functions of human colon during ageing are region-dependent.
Broad, John; Kung, Victor W S; Palmer, Alexandra; Elahi, Shezan; Karami, Azadeh; Darreh-Shori, Taher; Ahmed, Shafi; Thaha, Mohamed Adhnan; Carroll, Rebecca; Chin-Aleong, Joanne; Martin, Joanne E; Saffrey, M Jill; Knowles, Charles H; Sanger, Gareth John.
Afiliación
  • Broad J; Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
  • Kung VWS; Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
  • Palmer A; Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
  • Elahi S; Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
  • Karami A; Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, NEO, Stockholm, Sweden.
  • Darreh-Shori T; Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, NEO, Stockholm, Sweden.
  • Ahmed S; Department of Surgery, Barts Health NHS Trust, The Royal London Hospital, London, UK.
  • Thaha MA; Department of Pathology, Barts Health NHS Trust, The Royal London Hospital, London, UK.
  • Carroll R; Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
  • Chin-Aleong J; Department of Surgery, Barts Health NHS Trust, The Royal London Hospital, London, UK.
  • Martin JE; Department of Pathology, Barts Health NHS Trust, The Royal London Hospital, London, UK.
  • Saffrey MJ; Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
  • Knowles CH; Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
  • Sanger GJ; Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
Gut ; 68(7): 1210-1223, 2019 07.
Article en En | MEDLINE | ID: mdl-30228216
OBJECTIVE: To determine if human colonic neuromuscular functions decline with increasing age. DESIGN: Looking for non-specific changes in neuromuscular function, a standard burst of electrical field stimulation (EFS) was used to evoke neuronally mediated (cholinergic/nitrergic) contractions/relaxations in ex vivomuscle strips of human ascending and descending colon, aged 35-91 years (macroscopically normal tissue; 239 patients undergoing cancer resection). Then, to understand mechanisms of change, numbers and phenotype of myenteric neurons (30 306 neurons stained with different markers), densities of intramuscular nerve fibres (51 patients in total) and pathways involved in functional changes were systematically investigated (by immunohistochemistry and use of pharmacological tools) in elderly (≥70 years) and adult (35-60 years) groups. RESULTS: With increasing age, EFS was more likely to evoke muscle relaxation in ascending colon instead of contraction (linear regression: n=109, slope 0.49%±0.21%/year, 95% CI), generally uninfluenced by comorbidity or use of medications. Similar changes were absent in descending colon. In the elderly, overall numbers of myenteric and neuronal nitric oxide synthase-immunoreactive neurons and intramuscular nerve densities were unchanged in ascending and descending colon, compared with adults. In elderly ascending, not descending, colon numbers of cell bodies exhibiting choline acetyltransferase immunoreactivity increased compared with adults (5.0±0.6 vs 2.4±0.3 neurons/mm myenteric plexus, p=0.04). Cholinergically mediated contractions were smaller in elderly ascending colon compared with adults (2.1±0.4 and 4.1±1.1 g-tension/g-tissue during EFS; n=25/14; p=0.04); there were no changes in nitrergic function or in ability of the muscle to contract/relax. Similar changes were absent in descending colon. CONCLUSION: In ascending not descending colon, ageing impairs cholinergic function.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colon Ascendente / Colon Descendente / Contracción Muscular / Fibras Nerviosas Límite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Gut Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colon Ascendente / Colon Descendente / Contracción Muscular / Fibras Nerviosas Límite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Gut Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido