Neural derivation of human dental pulp stem cells via neurosphere technique.
Bratisl Lek Listy
; 119(9): 550-553, 2018.
Article
en En
| MEDLINE
| ID: mdl-30226064
INTRODUCTION: Human dental pulp stem cells (hDPSCs) are multipotent stem cells providing an autologous noninvasive cell source. The study evaluates the neurogenic potential of hDPSCs using neural growth factor inducers and neurosphere technique. METHODS: The hDPSCs were differentiated into neurons using neural induction medium containing retinoic acid (RA). Neuroprogenitor cells were evaluated for nestin and NF68 using immunocytochemistry. The mature neuron markers, MAP2 and ß-tubulin, were investigated at the end stage of induction phase. RESULTS: The neuroprogenitor differentiation was confirmed by immunostaining for nestin and NF68 markers. The differentiated neurons were positive for specific neuron markers, namely for MAP2 and ß-tubulin. The results indicated that the neural differentiation medium and neurosphere technique improve the generation of neuroprogenitor cells as well as mature neurons via exhibiting specific neural markers, namely nestin, NF68, MAP2 and ß-tubulin. CONCLUSION: Our findings highlight the differentiation capacity of hDPSCs via neurosphere technique in the presence of neural inducers for mesenchymal stem cells. It is suggested that the neural differentiation potential of hDPSCs can be exploited as a source of stem cells for therapy of neurodegenerative diseases (Fig. 5, Ref. 20).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Madre
/
Diferenciación Celular
/
Separación Celular
/
Pulpa Dental
Límite:
Humans
Idioma:
En
Revista:
Bratisl Lek Listy
Año:
2018
Tipo del documento:
Article
Pais de publicación:
Eslovaquia