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Changes in the photosynthetic apparatus and lipid droplet formation in Chlamydomonas reinhardtii under iron deficiency.
Devadasu, Elsinraju; Chinthapalli, Dinesh Kumar; Chouhan, Nisha; Madireddi, Sai Kiran; Rasineni, Girish Kumar; Sripadi, Prabhakar; Subramanyam, Rajagopal.
Afiliación
  • Devadasu E; Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, Telangana, 500046, India.
  • Chinthapalli DK; Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, Telangana, 500046, India.
  • Chouhan N; Analytical Chemistry and Mass Spectrometry, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, 500 007, India.
  • Madireddi SK; Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, Telangana, 500046, India.
  • Rasineni GK; Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, Telangana, 500046, India.
  • Sripadi P; Center for Excellence in Medical Services Pvt. Ltd., Kineta Towers, Road No. 3, Banjara Hills, Hyderabad, Telangana, 500034, India.
  • Subramanyam R; Analytical Chemistry and Mass Spectrometry, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, 500 007, India.
Photosynth Res ; 139(1-3): 253-266, 2019 Mar.
Article en En | MEDLINE | ID: mdl-30218258
The unicellular photosynthetic alga Chlamydomonas reinhardtii was propagated in iron deficiency medium and patterns of growth, photosynthetic efficiency, lipid accumulation, as well as the expression of lipid biosynthetic and photosynthesis-related proteins were analysed and compared with iron-sufficient growth conditions. As expected, the photosynthetic rate was reduced (maximally after 4 days of growth) as a result of increased non-photochemical quenching (NPQ). Surprisingly, the stress-response protein LHCSR3 was expressed in conditions of iron deficiency that cause NPQ induction. In addition, the protein contents of both the PSI and PSII reaction centres were gradually reduced during growth in iron deficiency medium. Interestingly, the two generations of Fe deficiency cells could be able to recover the photosynthesis but the second generation cells recovered much slower as these cells were severely in shock. Analysis by flow cytometry with fluorescence-activated cell sorting and thin layer chromatography showed that iron deficiency also induced the accumulation of triacylglycerides (TAG), which resulted in the formation of lipid droplets. This was most significant between 48 and 72 h of growth. Dramatic increases in DGAT2A and PDAT1 levels were caused by iron starvation, which indicated that the biosynthesis of TAG had been increased. Analysis using gas chromatography mass spectrometry showed that levels of 16:0, 18:0, 18:2 and 18:3Δ9,12,15 fatty acids were significantly elevated. The results of this study highlight the genes/enzymes of Chlamydomonas that affect lipid synthesis through their influence on photosynthesis, and these represent potential targets of metabolic engineering to develop strains for biofuel production.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Chlamydomonas reinhardtii / Complejo de Proteína del Fotosistema II / Deficiencias de Hierro / Luz Idioma: En Revista: Photosynth Res Asunto de la revista: METABOLISMO Año: 2019 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Chlamydomonas reinhardtii / Complejo de Proteína del Fotosistema II / Deficiencias de Hierro / Luz Idioma: En Revista: Photosynth Res Asunto de la revista: METABOLISMO Año: 2019 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos