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Protective effect of Thai silk extracts on drug-induced phototoxicity in human epidermal A431 cells and a reconstructed human epidermis model.
Rosena, Apiwan; Koobkokkruad, Thongchai; Eaknai, Waleewan; Bunwatcharaphansakun, Phichaporn; Maniratanachote, Rawiwan; Aueviriyavit, Sasitorn.
Afiliación
  • Rosena A; Nano Safety and Risk Assessment Laboratory, National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park, Pathum Thani, Thailand.
  • Koobkokkruad T; Nano-Cosmeceuticals Laboratory, National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park, Pathum Thani, Thailand.
  • Eaknai W; Nano-Cosmeceuticals Laboratory, National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park, Pathum Thani, Thailand.
  • Bunwatcharaphansakun P; Nano-Cosmeceuticals Laboratory, National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park, Pathum Thani, Thailand.
  • Maniratanachote R; Nano Safety and Risk Assessment Laboratory, National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park, Pathum Thani, Thailand.
  • Aueviriyavit S; Nano Safety and Risk Assessment Laboratory, National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park, Pathum Thani, Thailand. Electronic address: sasitorn@nanotec.or.th.
J Photochem Photobiol B ; 188: 50-59, 2018 Nov.
Article en En | MEDLINE | ID: mdl-30216760
Bombyx mori silk extracts, derived from the cocoon degumming process of draw and dye silk in the textile industry, are mainly composed of sericin protein. To add value to the Thai silk extracts, and hence the silk industry, a simple enrichment process was recently developed and the enriched silk extracts were then applied in nano-cosmeceutical products and nano-delivery systems. In this study, the protective effect of Thai silk extracts from three different strains of Bombyx mori on the drug-induced phototoxicity was evaluated in vitro using chlorpromazine (CPZ), a commonly used antipsychotic drug, as a representative phototoxic drug. The human epidermal A431 cell line and reconstructed human epidermis (RhE) model were used as the in vitro skin model. The silk extracts significantly improved the viability of A431 cells after CPZ exposure and ultraviolet A (UVA) irradiation, as shown by the significantly increased CPZ and UVA IC50 values and the decreased proportion of apoptotic cells. The protective effect of these silk extracts against the CPZ-induced UVA-phototoxicity in A431 cells was associated with the attenuation of intracellular oxidative stress via an increased intracellular glutathione level. Likewise, the silk extracts exhibited a protective effect on the CPZ-induced UVA-phototoxicity in the RhE model, in terms of an improved tissue viability and attenuation of the released inflammatory cytokine, interleukin-1α. These findings support the potential usefulness of silk extracts in novel applications, especially in the protection of drug-induced phototoxicity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rayos Ultravioleta / Sustancias Protectoras / Seda / Epidermis Tipo de estudio: Prognostic_studies Límite: Animals / Humans País/Región como asunto: Asia Idioma: En Revista: J Photochem Photobiol B Asunto de la revista: BIOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rayos Ultravioleta / Sustancias Protectoras / Seda / Epidermis Tipo de estudio: Prognostic_studies Límite: Animals / Humans País/Región como asunto: Asia Idioma: En Revista: J Photochem Photobiol B Asunto de la revista: BIOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Suiza