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In situ detection of CD73+ CD90+ CD105+ lineage: Mesenchymal stromal cells in human placenta and bone marrow specimens by chipcytometry.
Consentius, Christine; Mirenska, Anja; Jurisch, Anke; Reinke, Simon; Scharm, Markus; Zenclussen, Ana Claudia; Hennig, Christian; Volk, Hans-Dieter.
Afiliación
  • Consentius C; Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Berlin, Germany.
  • Mirenska A; Berlin-Brandenburg School for Regenerative Therapies (BSRT), Berlin, Germany.
  • Jurisch A; Zellkraftwerk, Hannover, Germany.
  • Reinke S; Institute of Medical Immunology, Berlin, Germany.
  • Scharm M; Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Berlin, Germany.
  • Zenclussen AC; Experimental Obstetrics and Gynaecology, Medical Faculty, Otto-von-Guericke-University, Magdeburg, Germany.
  • Hennig C; Experimental Obstetrics and Gynaecology, Medical Faculty, Otto-von-Guericke-University, Magdeburg, Germany.
  • Volk HD; Zellkraftwerk, Hannover, Germany.
Cytometry A ; 93(9): 889-893, 2018 07.
Article en En | MEDLINE | ID: mdl-30211969
Mesenchymal stromal cells (MSCs) support endogenous regeneration and present therefore promising opportunities for in situ tissue engineering. They can be isolated and expanded from various tissues, for example, bone marrow, adipose tissue, or placenta. The minimal consensus definition criteria of ex vivo expanded MSCs requires them to be positive for CD73, CD90, and CD105 expression, while being negative for CD34, CD45, CD14, CD19, and HLA-DR. This study aimed to compare the in situ phenotype of MSCs with that of their culture-expanded progeny. We report for the first time in situ detection of cells expressing this marker combination in human placenta cryosections as well as in bone marrow aspirates using multiplex-immunohistology (Chipcytometry), a technique that allows staining of more than 100 biomarkers consecutively on the same cell. © 2018 International Society for Advancement of Cytometry.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Médula Ósea / Células de la Médula Ósea / 5'-Nucleotidasa / Antígenos Thy-1 / Células Madre Mesenquimatosas / Endoglina Tipo de estudio: Diagnostic_studies Límite: Female / Humans / Pregnancy Idioma: En Revista: Cytometry A Año: 2018 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Médula Ósea / Células de la Médula Ósea / 5'-Nucleotidasa / Antígenos Thy-1 / Células Madre Mesenquimatosas / Endoglina Tipo de estudio: Diagnostic_studies Límite: Female / Humans / Pregnancy Idioma: En Revista: Cytometry A Año: 2018 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos