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Biomimetic oyster shell-replicated topography alters the behaviour of human skeletal stem cells.
Waddell, Shona J; de Andrés, María C; Tsimbouri, Penelope M; Alakpa, Enateri V; Cusack, Maggie; Dalby, Matthew J; Oreffo, Richard Oc.
Afiliación
  • Waddell SJ; Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, Faculty of Medicine, University of Southampton, Southampton, UK.
  • de Andrés MC; Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, Faculty of Medicine, University of Southampton, Southampton, UK.
  • Tsimbouri PM; Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, CMVLS, University of Glasgow, Glasgow, UK.
  • Alakpa EV; Department of Integrative Medical Biology, Umeå University, Umeå, Sweden.
  • Cusack M; Division of Biological and Environmental Science, University of Stirling, Stirling, UK.
  • Dalby MJ; Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, CMVLS, University of Glasgow, Glasgow, UK.
  • Oreffo RO; Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, Faculty of Medicine, University of Southampton, Southampton, UK.
J Tissue Eng ; 9: 2041731418794007, 2018.
Article en En | MEDLINE | ID: mdl-30202512
The regenerative potential of skeletal stem cells provides an attractive prospect to generate bone tissue needed for musculoskeletal reparation. A central issue remains efficacious, controlled cell differentiation strategies to aid progression of cell therapies to the clinic. The nacre surface from Pinctada maxima shells is known to enhance bone formation. However, to date, there is a paucity of information on the role of the topography of P. maxima surfaces, nacre and prism. To investigate this, nacre and prism topographical features were replicated onto polycaprolactone and skeletal stem cell behaviour on the surfaces studied. Skeletal stem cells on nacre surfaces exhibited an increase in cell area, increase in expression of osteogenic markers ALP (p < 0.05) and OCN (p < 0.01) and increased metabolite intensity (p < 0.05), indicating a role of nacre surface to induce osteogenic differentiation, while on prism surfaces, skeletal stem cells did not show alterations in cell area or osteogenic marker expression and a decrease in metabolite intensity (p < 0.05), demonstrating a distinct role for the prism surface, with the potential to maintain the skeletal stem cell phenotype.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Tissue Eng Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Tissue Eng Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido