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Enzyme-assisted extraction and liquid chromatography-inductively coupled plasma mass spectrometry for the determination of arsenic species in fish.
Zhao, Fa; Liu, Yanming; Zhang, Xiqi; Dong, Rui; Yu, Wenjiang; Liu, Yanfang; Guo, Zhimou; Liang, Xinmiao; Zhu, Jianhua.
Afiliación
  • Zhao F; Shandong Institute for Food and Drug Control, PR China. Electronic address: zhaofa1987@126.com.
  • Liu Y; Shandong Institute for Food and Drug Control, PR China. Electronic address: msymliu@163.com.
  • Zhang X; Shandong Institute for Food and Drug Control, PR China.
  • Dong R; Shandong Institute for Food and Drug Control, PR China.
  • Yu W; Shandong Institute for Food and Drug Control, PR China.
  • Liu Y; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, PR China.
  • Guo Z; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, PR China.
  • Liang X; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, PR China.
  • Zhu J; Shandong Institute for Food and Drug Control, PR China.
J Chromatogr A ; 1573: 48-58, 2018 Oct 26.
Article en En | MEDLINE | ID: mdl-30195857
A sensitive, simple and rapid method for the simultaneous determination of eleven arsenic species has been developed. A high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) technique was used for the analysis of eleven arsenic species in less than 17 min. Different extraction solutions were explored and the recovery results using water and aqueous acidic solvents, aqueous organic solvents and enzymes showed that 40 mg protease with 0.75 mL 0.5% hydrochloric acid (v/v) as the extraction agent gave the best experimental results. Species separation with ammonium carbonate solution as the mobile phase was conducted on an anion-exchange chromatographic column using gradient elution. The column temperature was 20 °C and kinetic energy discrimination (KED) was employed to eliminate spectral interference. The use of KED mode effectively removed interference from 75ArCl. The detection limit (LD) of the method was in the range of 0.11-0.59 µg kg-1. Repeatability values obtained for spiked real fish samples were in the range of 1.1%-7.6%. Accuracy was calculated based on the analysis of spiked real fish samples at five concentration levels. Obtained recoveries were 91%-106%. The validated method was used in a pilot study to analyze real samples of fish, the organic arsenic especially AsB was the major arsenic specie present in the analyzed samples, only trace amount of inorganic arsenic were detected. The analytical method should improve the assessment of human exposure associated with arsenic intake from fish.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arsénico / Espectrometría de Masas / Cromatografía Líquida de Alta Presión / Peces / Análisis de los Alimentos Límite: Animals / Humans Idioma: En Revista: J Chromatogr A Año: 2018 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arsénico / Espectrometría de Masas / Cromatografía Líquida de Alta Presión / Peces / Análisis de los Alimentos Límite: Animals / Humans Idioma: En Revista: J Chromatogr A Año: 2018 Tipo del documento: Article Pais de publicación: Países Bajos