Isolation of cDNA clones derived from a cellular gene transcriptionally induced by herpes simplex virus.
Nucleic Acids Res
; 14(14): 5629-40, 1986 Jul 25.
Article
en En
| MEDLINE
| ID: mdl-3016658
A small number of cellular proteins accumulate to high levels in cells infected with Herpes Simplex Virus (HSV) despite a generalised repression of most host cell bio-synthesis. An antibody to one such protein has been used to screen a lambda gt11 library and for polysome immunoprecipitation in order to isolate cDNA clones derived from the corresponding gene. The cDNA clones have been used in dot blot and nuclear run-off assays to show that HSV, like other DNA tumour viruses can transcriptionally induce a cellular gene. The mechanism of this effect which is dependent on viral protein synthesis and its possible significance in transformation by HSV are discussed.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transcripción Genética
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ADN
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Transformación Celular Neoplásica
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Clonación Molecular
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Simplexvirus
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Genes
Límite:
Animals
Idioma:
En
Revista:
Nucleic Acids Res
Año:
1986
Tipo del documento:
Article
Pais de publicación:
Reino Unido