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Development of a multi-stage continuous fermentation strategy for docosahexaenoic acid production by Schizochytrium sp.
Guo, Dong-Sheng; Ji, Xiao-Jun; Ren, Lu-Jing; Yin, Feng-Wei; Sun, Xiao-Man; Huang, He; Zhen, Gao.
Afiliación
  • Guo DS; College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
  • Ji XJ; College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China; Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China.
  • Ren LJ; College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China; Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China.
  • Yin FW; College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
  • Sun XM; College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
  • Huang H; School of Pharmaceutical Sciences, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China; State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, No. 5 Xinmofan Road, Nanjing 210009, People's Republic of China; Jiangsu National
  • Zhen G; College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China. Electronic address: gaozhen@njtech.edu.cn.
Bioresour Technol ; 269: 32-39, 2018 Dec.
Article en En | MEDLINE | ID: mdl-30149252
Docosahexaenoic acid (DHA) has wide-ranging benefits for normal development of the visual and nervous systems in infants. A sustainable source of DHA production through fermentation using Schizochytrium sp. has been developed. In this paper, we present the discovery of growth-uncoupled DHA production by Schizochytrium sp. and the development of corresponding kinetic models of fed-batch fermentations, which can be used to describe and predict the cell growth and substrate utilization as well as lipid and DHA production. Based on this kinetic model, a predictive model of multi-stage continuous fermentation process was established and used to analyze, optimize and design the process parameters. Optimal predicted processes of two-stage and three-stage continuous fermentation were developed and verified in lab-scale bioreactor based on the predicted process parameters. A successful three-stage continuous fermentation was achieved, which increased the lipid, DHA content and DHA productivity by 47.6, 64.3 and 97.1%, respectively, compared with two-stage continuous fermentation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácidos Docosahexaenoicos / Estramenopilos / Fermentación Tipo de estudio: Prognostic_studies Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácidos Docosahexaenoicos / Estramenopilos / Fermentación Tipo de estudio: Prognostic_studies Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido