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Autophagy is required for proper meiosis of porcine oocytes maturing in vitro.
Shen, Xing-Hui; Jin, Yong-Xun; Liang, Shuang; Kwon, Jeong-Woo; Zhu, Ji-Wei; Lei, Lei; Kim, Nam-Hyung.
Afiliación
  • Shen XH; Department of Histology and Embryology, Harbin Medical University, Harbin, Heilongjiang, China.
  • Jin YX; Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
  • Liang S; Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
  • Kwon JW; Department of Animal Science, College of Animal Sciences, Jilin University, Changchun, Jilin, China.
  • Zhu JW; Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
  • Lei L; Department of Animal Science, College of Animal Sciences, Jilin University, Changchun, Jilin, China.
  • Kim NH; Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
Sci Rep ; 8(1): 12581, 2018 08 22.
Article en En | MEDLINE | ID: mdl-30135500
Autophagy is an essential cellular mechanism that degrades cytoplasmic proteins and organelles to recycle their components; however, the contribution of autophagy during meiosis has not been studied in porcine oocytes maturing in vitro. In this study, we observed that the autophagy-related gene, LC3, was expressed in porcine oocytes during maturation for 44 h in vitro. Knockdown of the autophagy-related gene, BECN1, reduced both BECN1 and LC3 protein expression levels. Moreover, BECN1 knockdown and treatment with the autophagy inhibitor, LY294002, during maturation of porcine oocytes in vitro impaired polar body extrusion, disturbed mitochondrial function, triggered the DNA damage response, and induced early apoptosis in porcine oocytes. Autophagy inhibition during oocyte maturation also impaired the further developmental potential of porcine oocytes. These results indicate that autophagy is required for the in vitro maturation of porcine oocytes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Autofagia / Meiosis Límite: Animals Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Autofagia / Meiosis Límite: Animals Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido