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Formulation and in vitro evaluation of magnetoliposomes as a potential nanotool in colorectal cancer therapy.
Lorente, Cristina; Cabeza, Laura; Clares, Beatriz; Ortiz, Raúl; Halbaut, Lyda; Delgado, Ángel V; Perazzoli, Gloria; Prados, José; Arias, José L; Melguizo, Consolación.
Afiliación
  • Lorente C; Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, Spain.
  • Cabeza L; Biosanitary Institute of Granada (ibs.GRANADA), Andalusian Health Service (SAS), University of Granada, Spain; Institute of Biopathology and Regenerative Medicine (IBIMER), Biomedical Research Centre (CIBM), University of Granada, Spain; Department of Human Anatomy and Embryology, University of Gran
  • Clares B; Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, Spain; Biosanitary Institute of Granada (ibs.GRANADA), Andalusian Health Service (SAS), University of Granada, Spain; Institute of Nanoscience and Nanotechnology (IN2UB), University of Barcelona, Spain.
  • Ortiz R; Institute of Biopathology and Regenerative Medicine (IBIMER), Biomedical Research Centre (CIBM), University of Granada, Spain; Department of Human Anatomy and Embryology, University of Granada, Spain.
  • Halbaut L; Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, University of Barcelona, Spain.
  • Delgado ÁV; Department of Applied Physics, Faculty of Sciences, University of Granada, Spain.
  • Perazzoli G; Biosanitary Institute of Granada (ibs.GRANADA), Andalusian Health Service (SAS), University of Granada, Spain; Institute of Biopathology and Regenerative Medicine (IBIMER), Biomedical Research Centre (CIBM), University of Granada, Spain.
  • Prados J; Biosanitary Institute of Granada (ibs.GRANADA), Andalusian Health Service (SAS), University of Granada, Spain; Institute of Biopathology and Regenerative Medicine (IBIMER), Biomedical Research Centre (CIBM), University of Granada, Spain; Department of Human Anatomy and Embryology, University of Gran
  • Arias JL; Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, Spain; Biosanitary Institute of Granada (ibs.GRANADA), Andalusian Health Service (SAS), University of Granada, Spain; Institute of Biopathology and Regenerative Medicine (IBIMER), Biomedical Research Ce
  • Melguizo C; Biosanitary Institute of Granada (ibs.GRANADA), Andalusian Health Service (SAS), University of Granada, Spain; Institute of Biopathology and Regenerative Medicine (IBIMER), Biomedical Research Centre (CIBM), University of Granada, Spain; Department of Human Anatomy and Embryology, University of Gran
Colloids Surf B Biointerfaces ; 171: 553-565, 2018 Nov 01.
Article en En | MEDLINE | ID: mdl-30096477
Magnetoliposomes (MLPs) offer many new possibilities in cancer therapy and diagnosis, including the transport of antitumor drugs, hyperthermia treatment, detection using imaging techniques, and even cell migration. However, high biocompatibility and functionality after cell internalization are essential to their successful application. We synthesized maghemite nanoparticles (γ-Fe2O3) by oxidizing magnetite cores (Fe3O4) and coating them with phosphatidylcholine (PC) liposomes, obtained using the thin film hydration method, to generate MLPs. The MLPs were tested in vitro, using human tumor and non-tumor colon cell lines, for cytotoxicity, cell uptake and cellular distribution, and magnetically-induced cell mobility. In addition, blood cells biocompatibility studies were performed. The mean size of the MLPs, with a core of γ-Fe2O3 completely surrounded by PC liposomes, was 90 ± 20 nm, showing a soft magnetic character and a great biocompatibility in all the cell lines assayed including blood cells. Prussian blue staining showed a high MLP cell uptake with maximum internalization at 24 h. TEM analysis showed the MLPs surrounded by the cell membrane and in the cell periphery, suggesting internalization by endocytosis and/or macropinocytosis. Interestingly, the mitochondria presented MLP accumulations, particularly in tumor cells. Finally, MLPs within colon cancer cells were able to induce cell migration when a magnetic field was applied in vitro, indicating the functionality of our nanoformulation. A promising biomedical application of these MLPs is anticipated based on their physical, chemical and biological properties.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatidilcolinas / Compuestos Férricos / Neoplasias Colorrectales / Nanotecnología / Nanopartículas de Magnetita / Antineoplásicos Límite: Animals / Humans Idioma: En Revista: Colloids Surf B Biointerfaces Asunto de la revista: QUIMICA Año: 2018 Tipo del documento: Article País de afiliación: España Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatidilcolinas / Compuestos Férricos / Neoplasias Colorrectales / Nanotecnología / Nanopartículas de Magnetita / Antineoplásicos Límite: Animals / Humans Idioma: En Revista: Colloids Surf B Biointerfaces Asunto de la revista: QUIMICA Año: 2018 Tipo del documento: Article País de afiliación: España Pais de publicación: Países Bajos