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Pericytes reduce inflammation and collagen deposition in acute wounds.
Bodnar, Richard J; Yang, Tianbing; Rigatti, Lora H; Liu, Fang; Evdokiou, Alexander; Kathju, Sandeep; Satish, Latha.
Afiliación
  • Bodnar RJ; Veterans Affairs Medical Center, Pittsburgh, Pennsylvania, USA; Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
  • Yang T; Department of Ophthalmology, Pittsburgh, Pennsylvania, USA.
  • Rigatti LH; Division of Laboratory Animal Resources, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
  • Liu F; Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
  • Evdokiou A; Research Department, Shriners Hospitals for Children, Cincinnati, Ohio, USA.
  • Kathju S; McGowan Institute for Regenerative Medicine, Pittsburgh, Pennsylvania, USA.
  • Satish L; Research Department, Shriners Hospitals for Children, Cincinnati, Ohio, USA; Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio, USA; Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA. Electronic address: lsatish@shrinenet
Cytotherapy ; 20(8): 1046-1060, 2018 08.
Article en En | MEDLINE | ID: mdl-30093323
BACKGROUND: Pericytes have been shown to have mesenchymal stromal cell-like properties and play a role in tissue regeneration. The goal of this study was to determine whether the addition of a pericyte sheet to a full-thickness dermal wound would enhance the healing of an acute wound. METHODS: Human muscle-derived pericytes and human dermal fibroblasts were formed into cell sheets, then applied to full-thickness excisional wounds on the dorsum of nu/nu mice. Histology was performed to evaluate epidermal and dermal reformation, inflammation and fibrosis. In addition, real-time reverse transcriptase-polymerase chain reaction (RT-PCR) was used to determine cytokine response. RESULTS: Pericytes were detected in the wounds until day 16 but not fibroblasts. Decrease in wound size was noted in pericyte sheet-treated wounds. Enhanced neo-vascularization and healthy granulation tissue formation were noted in the pericyte-treated wounds. Expression of type I collagen messenger RNA (mRNA) was significantly higher in the fibroblast-treated group, whereas Type III collagen mRNA showed significant increase in the pericyte group at days 3, 6 and 9 compared with the fibroblast and no-cell groups. Trichrome staining revealed thick unorganized collagen fibrils in the fibroblast-treated wounds, whereas pericyte-treated wounds contained thinner and more alligned collagen fibrils. Tumor necrosis factor (TNF)-α mRNA levels were increased in the fibroblast-treated wounds compared with pericyte-treated wounds. DISCUSSION: The addition of pericytes may confer beneficial effects to wound healing resulting in reduced recruitment of inflammatory cells and collagen I deposition, potential to enhance wound closure and better collagen alignment promoting stronger tissue.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cicatrización de Heridas / Colágeno / Dermis / Pericitos / Inflamación Idioma: En Revista: Cytotherapy Asunto de la revista: TERAPEUTICA Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cicatrización de Heridas / Colágeno / Dermis / Pericitos / Inflamación Idioma: En Revista: Cytotherapy Asunto de la revista: TERAPEUTICA Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido