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Menthol ameliorates voiding dysfunction in types I and II diabetic mouse model.
de Oliveira, Mariana G; Nascimento, Daniel M; Alexandre, Eduardo C; Bonilla-Becerra, Sandra M; Zapparoli, Adriana; Mónica, Fabiola Z; Antunes, Edson.
Afiliación
  • de Oliveira MG; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas, Campinas, Sao Paulo, Brazil.
  • Nascimento DM; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas, Campinas, Sao Paulo, Brazil.
  • Alexandre EC; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas, Campinas, Sao Paulo, Brazil.
  • Bonilla-Becerra SM; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas, Campinas, Sao Paulo, Brazil.
  • Zapparoli A; Faculty of Medical Sciences, Department of Medicine and Experimental Surgery, University of Campinas, Campinas, Sao Paulo, Brazil.
  • Mónica FZ; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas, Campinas, Sao Paulo, Brazil.
  • Antunes E; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas, Campinas, Sao Paulo, Brazil.
Neurourol Urodyn ; 37(8): 2510-2518, 2018 11.
Article en En | MEDLINE | ID: mdl-30088676
AIMS: Overactive bladder (OAB) is one of the most common complications of both type 1 (T1DM) and type 2 diabetes mellitus (T2DM). In healthy conditions, menthol infused intravesically reduces the threshold for initiating micturition reflex, but no study evaluated its effects in diabetic conditions. Therefore, we have used mouse models of T1DM and T2DM to evaluate the effects of menthol on cystometric alterations and increased bladder contractility in vitro. METHODS: For T1DM induction, male C57BL6 mice were injected with streptozotocin (STZ) and evaluated after 4 weeks. For T2DM induction, mice were fed with high-fat diet (HFD) for 12 weeks to induce obesity. Urodynamic profiles were assessed by filling cystometry through the infusion of menthol (100 µM for 30 min) or vehicle (DMSO 0.1%). Contractile responses to carbachol, potassium chloride (KCl), and electrical-field stimulation (EFS) were measured in isolated bladders after 20 min incubation with menthol (100 µM) or vehicle. RESULTS: Filling cystometry showed that STZ-injected mice exhibited higher bladder capacity, threshold pressure, and non-voiding contractions (NVCs), which were significantly reduced by menthol infusion. The increased voiding frequency in STZ group were unaffected by menthol. In HFD-fed obese mice menthol significantly attenuated the increased threshold pressure and NVC frequency, but unaffected the changes of voiding frequency. In both STZ-injected and HFD-fed mice, incubation of isolated bladders with menthol normalized the enhanced contractile responses to carbachol, KCl, and EFS stimulation. CONCLUSIONS: Menthol may be a potential pharmacological option for the treatment of OAB as a consequence of T1DM and T2DM.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trastornos Urinarios / Diabetes Mellitus Tipo 1 / Diabetes Mellitus Tipo 2 / Vejiga Urinaria Hiperactiva / Mentol Límite: Animals Idioma: En Revista: Neurourol Urodyn Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trastornos Urinarios / Diabetes Mellitus Tipo 1 / Diabetes Mellitus Tipo 2 / Vejiga Urinaria Hiperactiva / Mentol Límite: Animals Idioma: En Revista: Neurourol Urodyn Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos