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A mouse model reproducing the pathophysiology of neonatal group B streptococcal infection.
Andrade, Elva Bonifácio; Magalhães, Ana; Puga, Ana; Costa, Madalena; Bravo, Joana; Portugal, Camila Cabral; Ribeiro, Adília; Correia-Neves, Margarida; Faustino, Augusto; Firon, Arnaud; Trieu-Cuot, Patrick; Summavielle, Teresa; Ferreira, Paula.
Afiliación
  • Andrade EB; ICBAS-Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, 4150-313, Porto, Portugal.
  • Magalhães A; i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135, Porto, Portugal.
  • Puga A; IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, 4200-135, Porto, Portugal.
  • Costa M; ESS-Escola Superior de Saúde, Instituto Politécnico do Porto, 4200-072, Porto, Portugal.
  • Bravo J; i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135, Porto, Portugal.
  • Portugal CC; IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, 4200-135, Porto, Portugal.
  • Ribeiro A; ICBAS-Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, 4150-313, Porto, Portugal.
  • Correia-Neves M; ICBAS-Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, 4150-313, Porto, Portugal.
  • Faustino A; ESS-Escola Superior de Saúde, Instituto Politécnico do Porto, 4200-072, Porto, Portugal.
  • Firon A; UMIB-Unit for Multidisciplinary Investigation in Biomedicine (Endocrine, Cardiovascular & Metabolic Research), University of Porto, 4150-313, Porto, Portugal.
  • Trieu-Cuot P; ICBAS-Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, 4150-313, Porto, Portugal.
  • Summavielle T; i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135, Porto, Portugal.
  • Ferreira P; IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, 4200-135, Porto, Portugal.
Nat Commun ; 9(1): 3138, 2018 08 07.
Article en En | MEDLINE | ID: mdl-30087335
Group B streptococcal (GBS) meningitis remains a devastating disease. The absence of an animal model reproducing the natural infectious process has limited our understanding of the disease and, consequently, delayed the development of effective treatments. We describe here a mouse model in which bacteria are transmitted to the offspring from vaginally colonised pregnant females, the natural route of infection. We show that GBS strain BM110, belonging to the CC17 clonal complex, is more virulent in this vertical transmission model than the isogenic mutant BM110∆cylE, which is deprived of hemolysin/cytolysin. Pups exposed to the more virulent strain exhibit higher mortality rates and lung inflammation than those exposed to the attenuated strain. Moreover, pups that survive to BM110 infection present neurological developmental disability, revealed by impaired learning performance and memory in adulthood. The use of this new mouse model, that reproduces key steps of GBS infection in newborns, will promote a better understanding of the physiopathology of GBS-induced meningitis.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Estreptocócicas / Transmisión Vertical de Enfermedad Infecciosa / Modelos Animales de Enfermedad Límite: Animals / Pregnancy Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2018 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Estreptocócicas / Transmisión Vertical de Enfermedad Infecciosa / Modelos Animales de Enfermedad Límite: Animals / Pregnancy Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2018 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Reino Unido