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Distribution of Virulence Genes among Salmonella Serotypes Isolated from Pigs in Southern Vietnam.
Lan, Tran T Q; Gaucher, Marie-Lou; Nhan, Nguyen T M; Letellier, Ann; Quessy, Sylvain.
Afiliación
  • Lan TTQ; 1 Department of Veterinary Biosciences, Faculty of Animal Science and Veterinary Medicine, Nông Lâm University, Ho Chi Minh City, Vietnam.
  • Gaucher ML; 2 Department of Pathology and Microbiology, University of Montreal, St-Hyacinthe, Quebec, Canada J2S 2M2.
  • Nhan NTM; 1 Department of Veterinary Biosciences, Faculty of Animal Science and Veterinary Medicine, Nông Lâm University, Ho Chi Minh City, Vietnam.
  • Letellier A; 2 Department of Pathology and Microbiology, University of Montreal, St-Hyacinthe, Quebec, Canada J2S 2M2.
  • Quessy S; 2 Department of Pathology and Microbiology, University of Montreal, St-Hyacinthe, Quebec, Canada J2S 2M2.
J Food Prot ; 81(9): 1459-1466, 2018 09.
Article en En | MEDLINE | ID: mdl-30084656
This study was carried out to evaluate the prevalence of Salmonella serogroups and serotypes and their virulence gene carriage in pig fecal samples from farms and slaughterhouses in some southern provinces of Vietnam. The presence of Salmonella was assessed based on culture enrichment of the collected samples and biochemical and serological analyses; 27.7% (51) of 184 samples were posititve for Salmonella. Based on the availability of antisera, serogroups were determined for 61% (31) of 51 isolates. Twenty isolates belonging to Salmonella serotypes Typhimurium (10 isolates), Anatum (8 isolates), Senftenberg (7 isolates), Paratyphi B (3 isolates), Paratyphi A (1 isolate), Montevideo (1 isolate), and Saintpaul (1 isolate) were further characterized by a multiplex PCR protocol targeting Salmonella invasion A and virulence plasmid C genes ( invA and spvC, respectively). Individual PCR assays were developed to detect genes for Salmonella enterotoxin ( stn) , Salmonella outer protein B ( sopB), and Salmonella fimbriae ( pef). Various carriage patterns were identified among tested isolates. The invA and sopB genes were found in all isolates, and the stn gene was found in 95% of the isolates. The spvC gene was found in only 5% of the Salmonella Typhimurium isolates. None of the isolates were positive for the pef gene. Among all isolates, the predominant genotypic virulence profile (virulotype) was characterized by the concomitant presence of invA, sopB, and stn in carrier strains. In contrast, two virulotypes comprising either invA, sopB, spvC, and stn or invA and sopB were identified for the Salmonella Typhimurium isolates. Virulotypes made up of multiple virulence genes were predominant in most Salmonella strains tested in this study, indicating that pigs might act as a reservoir for these virulent strains.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmonella / Porcinos / Factores de Virulencia / Heces Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals País/Región como asunto: Asia Idioma: En Revista: J Food Prot Año: 2018 Tipo del documento: Article País de afiliación: Vietnam Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmonella / Porcinos / Factores de Virulencia / Heces Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals País/Región como asunto: Asia Idioma: En Revista: J Food Prot Año: 2018 Tipo del documento: Article País de afiliación: Vietnam Pais de publicación: Estados Unidos