The Comparative Sensitivity of Immunohistochemical Markers of Megakaryocytic Differentiation in Acute Megakaryoblastic Leukemia.
Am J Clin Pathol
; 150(5): 461-467, 2018 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-30052718
OBJECTIVES: Immunohistochemistry (IHC) staining of core biopsy sections often plays an essential role in the diagnosis of acute megakaryoblastic leukemia (AMKL). The goal of this study was to define the relative sensitivities of commonly used stains for markers of megakaryocytic differentiation. METHODS: The sensitivities of IHC stains for CD42b, CD61, and von Willebrand factor (vWF) were compared in 32 cases of pediatric AMKL. RESULTS: The sensitivities of CD42b, CD61, and vWF were 90.6%, 78.1% and 62.5%, respectively. When CD42b and CD61 were used together, the combined sensitivity increased to 93.6%. There were no cases in which vWF was positive when both CD42b and CD61 were negative. CONCLUSIONS: CD42b can reliably be used as a solitary first-line marker for blasts of megakaryocytic lineage, whereas CD61 may be reserved for infrequent cases that are CD42b negative. There is no role for the routine use of vWF when CD42b and CD61 are available.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Leucemia Megacarioblástica Aguda
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Complejo GPIb-IX de Glicoproteína Plaquetaria
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Integrina beta3
Tipo de estudio:
Diagnostic_studies
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Evaluation_studies
Límite:
Child
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Humans
Idioma:
En
Revista:
Am J Clin Pathol
Año:
2018
Tipo del documento:
Article
Pais de publicación:
Reino Unido