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The identification of novel single nucleotide polymorphisms to assist in mapping the spread of Bacillus anthracis across the Southern Caucasus.
Sahin, Mitat; Buyuk, Fatih; Baillie, Les; Wölfel, Roman; Kotorashvili, Adam; Rehn, Alexandra; Antwerpen, Markus; Grass, Gregor.
Afiliación
  • Sahin M; Kafkas University, Kars, Turkey.
  • Buyuk F; Kafkas University, Kars, Turkey.
  • Baillie L; Cardiff University, Cardiff, Wales, UK.
  • Wölfel R; Bundeswehr Institute of Microbiology, Munich, Germany.
  • Kotorashvili A; Lugar Center for Public Health Research at the National Center for Disease Control, Tbilisi, Georgia.
  • Rehn A; Bundeswehr Institute of Microbiology, Munich, Germany.
  • Antwerpen M; Bundeswehr Institute of Microbiology, Munich, Germany.
  • Grass G; Bundeswehr Institute of Microbiology, Munich, Germany. gregorgrass@bundeswehr.org.
Sci Rep ; 8(1): 11254, 2018 07 26.
Article en En | MEDLINE | ID: mdl-30050151
Anthrax is common as a zoonotic disease in the southern Caucasus area including parts of Turkey and Georgia. In this region, population genetics of the etiological agent Bacillus anthracis comprises, where known, the major canonical single nucleotide polymorphism (canSNP) groups A.Br.Aust94 and A.Br.008/009 of the pathogen's global phylogeny, respectively. Previously, isolates of B. anthracis from Turkey have been genotyped predominantly by multi locus variable number of tandem repeat analysis (MLVA) or canSNP typing. While whole genome sequencing is the future gold standard, it is currently still costly. For that reason we were interested in identifying novel SNPs which could assist in further distinguishing closely related isolates using low cost assay platforms. In this study we sequenced the genomes of seven B. anthracis strains collected from the Kars province of Eastern Anatolia in Turkey and discovered new SNPs which allowed us to assign these and other geographically related strains to three novel branches of the major A-branch canSNP-group (A.Br.) Aust94. These new branches were named Kafkas-Geo 1-3 and comprised isolates from the Kars region and the neighboring republic of Georgia suggesting a common ancestry. The novel SNPs identified in this study connect the population genetics of B. anthracis in the South Caucasus and Turkey and will likely assist efforts to map the spread of the pathogen across this region.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus anthracis / Polimorfismo de Nucleótido Simple / Tipificación Molecular / Técnicas de Genotipaje / Genotipo / Carbunco Tipo de estudio: Diagnostic_studies / Prognostic_studies País/Región como asunto: Asia Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus anthracis / Polimorfismo de Nucleótido Simple / Tipificación Molecular / Técnicas de Genotipaje / Genotipo / Carbunco Tipo de estudio: Diagnostic_studies / Prognostic_studies País/Región como asunto: Asia Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Reino Unido