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Inventory of ABC proteins and their putative role in salt and drug tolerance in Debaryomyces hansenii.
Wasi, Mohd; Khandelwal, Nitesh Kumar; Vishwakarma, Poonam; Lynn, Andrew M; Mondal, Alok K; Prasad, Rajendra.
Afiliación
  • Wasi M; School of life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
  • Khandelwal NK; School of life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
  • Vishwakarma P; School of Information Technology, Jawaharlal Nehru University, New Delhi 110067, India.
  • Lynn AM; School of Information Technology, Jawaharlal Nehru University, New Delhi 110067, India.
  • Mondal AK; School of life Sciences, Jawaharlal Nehru University, New Delhi 110067, India. Electronic address: akmondal@mail.jnu.ac.in.
  • Prasad R; Amity Institute of Biotechnology and Integrative Sciences and Health, Amity University Haryana, Amity Education Valley Gurgaon, 122413, India. Electronic address: rprasad@amity.ggn.edu.
Gene ; 676: 227-242, 2018 Nov 15.
Article en En | MEDLINE | ID: mdl-30025928
ATP-binding cassette (ABC) is one of the largest superfamily of proteins, which are ubiquitously present, performing variety of cellular functions. These proteins as drug transporters have been enticing substantial consideration because of their clinical importance. The present study focuses on genome wide identification of ABC proteins of an important halotolerant yeast Debaryomyces hansenii and explores their role in salt and drug tolerance. Our bioinformatics analysis identified a total of 30 putative ABC protein-coding genes whose expression at transcript level was confirmed by qRT-PCR. Our comparative phylogenetic analysis of nucleotide binding domains of D. hansenii and topology prediction categorized these proteins into six subfamilies; ABCB/MDR, ABCC/MRP, ABCD/ALDP, ABCF/YEF3, ABCE/RLI, and ABCG/PDR based on the nomenclature adopted by the Human Genome Organization (HUGO). Further, our transmembrane domain (TMD) predictions suggest that out of 30 ABC proteins, only 22 proteins possess either two or one TMD and hence are considered as membrane localized ABC proteins. Notably, our transcriptional dynamics of ABC proteins encoding genes following D. hansenii cells treatment with different salts and drugs concentrations illustrated variable transcriptional response of some of the genes, pointing to their role in salt and drug tolerance. This study first time provides a comprehensive inventory of the ABC proteins of a haploid D. hansenii which will be helpful for exploring their functional relevance.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transportadoras de Casetes de Unión a ATP / Farmacorresistencia Fúngica / Debaryomyces / Tolerancia a la Sal Tipo de estudio: Prognostic_studies Idioma: En Revista: Gene Año: 2018 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transportadoras de Casetes de Unión a ATP / Farmacorresistencia Fúngica / Debaryomyces / Tolerancia a la Sal Tipo de estudio: Prognostic_studies Idioma: En Revista: Gene Año: 2018 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos