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Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses.
Freire, Marjorie C L C; Pol-Fachin, Laércio; Coêlho, Danilo F; Viana, Isabelle F T; Magalhães, Tereza; Cordeiro, Marli T; Fischer, Nico; Loeffler, Felix F; Jaenisch, Thomas; Franca, Rafael F; Marques, Ernesto T A; Lins, Roberto D.
Afiliación
  • Freire MCLC; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife 50740-465, Brazil.
  • Pol-Fachin L; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife 50740-465, Brazil.
  • Coêlho DF; Department of Fundamental Chemistry, Federal University of Pernambuco, Recife 50740-670, Brazil.
  • Viana IFT; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife 50740-465, Brazil.
  • Magalhães T; Department of Fundamental Chemistry, Federal University of Pernambuco, Recife 50740-670, Brazil.
  • Cordeiro MT; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife 50740-465, Brazil.
  • Fischer N; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife 50740-465, Brazil.
  • Loeffler FF; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife 50740-465, Brazil.
  • Jaenisch T; Department of Infectious Diseases, Section Clinical Tropical Medicine, Heidelberg University Hospital, INF 324, Heidelberg 69120, Germany.
  • Franca RF; HEiKA - Heidelberg Karlsruhe Research Partnership, Heidelberg University, Karlsruhe Institute of Technology (KIT), Karlsruhe 76131, Germany.
  • Marques ETA; Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, Potsdam 14476, Germany.
  • Lins RD; HEiKA - Heidelberg Karlsruhe Research Partnership, Heidelberg University, Karlsruhe Institute of Technology (KIT), Karlsruhe 76131, Germany.
ACS Omega ; 2(7): 3913-3920, 2017 Jul 31.
Article en En | MEDLINE | ID: mdl-30023708
B-cell epitope sequences from Zika virus (ZIKV) NS1 protein have been identified using epitope prediction tools. Mapping these sequences onto the NS1 surface reveals two major conformational epitopes and a single linear one. Despite an overall average sequence identity of ca. 55% between the NS1 from ZIKV and the four dengue virus (DENV) serotypes, epitope sequences were found to be highly conserved. Nevertheless, nonconserved epitope-flanking residues are responsible for a dramatically divergent electrostatic surface potential on the epitope regions of ZIKV and DENV2 serotypes. These findings suggest that strategies for differential diagnostics on the basis of short linear NS1 sequences are likely to fail due to immunological cross-reactions. Overall, results provide the molecular basis of differential discrimination between Zika and DENVs by NS1 monoclonal antibodies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: ACS Omega Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: ACS Omega Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos