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FGF-2 combined with bilayer artificial dermis composed of collagen matrix prompts generation of fat pad in subcutis of mice.
Kakudo, Natsuko; Morimoto, Naoki; Ogawa, Takeshi; Taketani, Shigeru; Kusumoto, Kenji.
Afiliación
  • Kakudo N; Department of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1, Shin-machi, Hirakata, Osaka, 573-1010, Japan. kakudon@hirakata.kmu.ac.jp.
  • Morimoto N; Department of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1, Shin-machi, Hirakata, Osaka, 573-1010, Japan.
  • Ogawa T; Department of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1, Shin-machi, Hirakata, Osaka, 573-1010, Japan.
  • Taketani S; Department of Microbiology, Kansai Medical University, Osaka, Japan.
  • Kusumoto K; Department of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1, Shin-machi, Hirakata, Osaka, 573-1010, Japan.
Med Mol Morphol ; 52(2): 73-81, 2019 Jun.
Article en En | MEDLINE | ID: mdl-30022246
Fibroblast growth factor (FGF)-2 induces mitogenesis, angiogenesis and adipogenesis. In this study, the adipogenesis-inducing effects of FGF-2 combined with bilayer artificial dermis in mice were evaluated. FGF-2-impregnated bilayer artificial dermis composed of collagen matrix, PELNAC (Gunze Corp., Osaka, Japan) was implanted subcutaneously into the thoracic region of mice. At 1, 2, 3, and 4 weeks, samples were collected for H&E staining, von Willebrand factor immunostaining, and perilipin immunostaining to examine adipose tissue localization and angiogenesis. The collagen matrix-implanted group without the addition of FGF-2 was prepared as a control. At 2 weeks after the implantation of FGF-2 combined with dermal substitutes, adipocytes appeared in the collagen fibers. At 3-4 weeks, a fat pad was generated with neovascularization. The thickness of the fat pad had significantly increased at 2, 3, and 4 weeks. The remaining collagen was decreased by absorption over time. In the control group, no fat pad was newly formed. This study has identified a promising method to enhance adipogenic effects in the murine subcutis, representing a potential technique for soft tissue reconstruction.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tejido Adiposo / Factor 2 de Crecimiento de Fibroblastos / Colágeno / Dermis / Tejido Subcutáneo Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Med Mol Morphol Asunto de la revista: BIOLOGIA MOLECULAR / PATOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tejido Adiposo / Factor 2 de Crecimiento de Fibroblastos / Colágeno / Dermis / Tejido Subcutáneo Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Med Mol Morphol Asunto de la revista: BIOLOGIA MOLECULAR / PATOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón