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Core-shell patterning of synthetic hydrogels via interfacial bioorthogonal chemistry for spatial control of stem cell behavior.
Dicker, K T; Song, J; Moore, A C; Zhang, H; Li, Y; Burris, D L; Jia, X; Fox, J M.
Afiliación
  • Dicker KT; Department of Materials Science and Engineering , University of Delaware , DuPont Hall , Newark , DE 19716 , USA . Email: xjia@udel.edu ; Email: jmfox@udel.edu.
  • Song J; Department of Materials Science and Engineering , University of Delaware , DuPont Hall , Newark , DE 19716 , USA . Email: xjia@udel.edu ; Email: jmfox@udel.edu.
  • Moore AC; Department of Biomedical Engineering , University of Delaware , Colburn Lab , Newark , DE 19716 , USA.
  • Zhang H; Department of Chemistry and Biochemistry , University of Delaware , Brown Lab , Newark , DE 19716 , USA.
  • Li Y; Department of Chemistry and Biochemistry , University of Delaware , Brown Lab , Newark , DE 19716 , USA.
  • Burris DL; Department of Biomedical Engineering , University of Delaware , Colburn Lab , Newark , DE 19716 , USA.
  • Jia X; Department of Mechanical Engineering , University of Delaware , Spencer Lab , Newark , DE 19716 , USA.
  • Fox JM; Department of Materials Science and Engineering , University of Delaware , DuPont Hall , Newark , DE 19716 , USA . Email: xjia@udel.edu ; Email: jmfox@udel.edu.
Chem Sci ; 9(24): 5394-5404, 2018 Jun 28.
Article en En | MEDLINE | ID: mdl-30009011
A new technique is described for the patterning of cell-guidance cues in synthetic extracellular matrices (ECM) for tissue engineering applications. Using s-tetrazine modified hyaluronic acid (HA), bis-trans-cyclooctene (TCO) crosslinkers and monofunctional TCO conjugates, interfacial bioorthogonal crosslinking was used to covalently functionalize hydrogels as they were synthesized at the liquid-gel interface. Through temporally controlled introduction of TCO conjugates during the crosslinking process, the enzymatic degradability, cell adhesivity, and mechanical properties of the synthetic microenvironment can be tuned with spatial precision. Using human mesenchymal stem cells (hMSCs) and hydrogels with a core-shell structure, we demonstrated the ability of the synthetic ECM with spatially defined guidance cues to modulate cell morphology in a biomimetic fashion. This new method for the spatially resolved introduction of cell-guidance cues for the establishment of functional tissue constructs complements existing methods that require UV-light or specialized equipment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Chem Sci Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Chem Sci Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido