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Injectable chitosan-hydroxyapatite hydrogels promote the osteogenic differentiation of mesenchymal stem cells.
Ressler, Antonia; Ródenas-Rochina, Joaquín; Ivankovic, Marica; Ivankovic, Hrvoje; Rogina, Anamarija; Gallego Ferrer, Gloria.
Afiliación
  • Ressler A; Faculty of Chemical Engineering and Technology, University of Zagreb, Marulicev trg 19, p.p.177, HR-10001 Zagreb, Croatia. Electronic address: aressler@fkit.hr.
  • Ródenas-Rochina J; Centre for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain. Electronic address: joaroroc@etsia.upv.es.
  • Ivankovic M; Faculty of Chemical Engineering and Technology, University of Zagreb, Marulicev trg 19, p.p.177, HR-10001 Zagreb, Croatia. Electronic address: mivank@fkit.hr.
  • Ivankovic H; Faculty of Chemical Engineering and Technology, University of Zagreb, Marulicev trg 19, p.p.177, HR-10001 Zagreb, Croatia. Electronic address: hivan@fkit.hr.
  • Rogina A; Faculty of Chemical Engineering and Technology, University of Zagreb, Marulicev trg 19, p.p.177, HR-10001 Zagreb, Croatia. Electronic address: arogina@fkit.hr.
  • Gallego Ferrer G; Centre for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain; Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Valencia, Spain. Electronic address: ggallego@ter.upv.es.
Carbohydr Polym ; 197: 469-477, 2018 Oct 01.
Article en En | MEDLINE | ID: mdl-30007636
Injectable hydrogels have emerged as promising biomaterials for tissue engineering applications. The goal of this study was to evaluate the potential of a pH-responsive chitosan-hydroxyapatite hydrogel to be used as a three-dimensional support for encapsulated mesenchymal stem cells (MSCs) osteogenic differentiation. In vitro enzymatic degradation of the hydrogel, during 28 days of incubation, in simulated physiological condiditons, was characterized by swelling measurements, molecular weight determination and SEM analysis of hydrogel microstructure. Osteogenic differentiation of encapsulated MSCs was confirmed by osteogenic Runx2, collagen type I and osteocalcin immunostaining and alkaline phosphatase quantification. The deposition of late osteogenic markers (calcium phosphates) detected by Alizarin red and von Kossa staining indicated an extracellular matrix mineralization.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Durapatita / Hidrogeles / Quitosano / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: Carbohydr Polym Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Durapatita / Hidrogeles / Quitosano / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: Carbohydr Polym Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido