LncRNA-SNHG7 regulates proliferation, apoptosis and invasion of bladder cancer cells assurance guidelines.

Zhong, Xintai; Long, Zhaolin; Wu, Shihao; Xiao, Meiling; Hu, Weilie

Zhong, Xintai; Long, Zhaolin; Wu, Shihao; Xiao, Meiling; Hu, Weilie.

Afiliación

Zhong X; Southern Medical University, Guangzhou, Guangdong, China; 2Department of Urology, Shunde Hospital of Southern Medical University, Foshan, Guangdong, China.

J BUON ; 23(3): 776-781, 2018.

Article en En | MEDLINE | ID: mdl-30003751

RESUMEN

PURPOSE: The purpose of this study was to investigate the expression of long-chain non-coding RNA (lncRNA) SNHG7 in bladder cancer tissues and cell lines, and to explore its impact on bladder cancer cell proliferation, apoptosis and invasion processes. METHODS: Bladder cancer tissues and near-cancer tissues were collected. The expression of lncRNA-SNHG7 in tissues and cell lines was detected by real-time PCR (RT-PCR). The expression of lncRNA-SNHG7 was downregulated by RNA interference (siRNA) as detected by RT-PCR that was used to detect the interference efficiency. CCK-8, flow cytometry and Transwell assays were used to evaluate the effect of lncRNASNHG7 on the proliferation, apoptosis and invasion capability of bladder cancer cells. The downregulation effect of lncRNA-SNHG7 on Epithelial-Mesenchymal Transition (EMT) related markers was tested by westernblot. RESULTS: lncRNA-SNHG7 was upregulated in bladder cancer cell lines. After the expression of lncRNA-SNHG7 was downregulated, the proliferation of bladder cancer cells was decreased, the apoptosis was increased, and the invasion ability of cells was decreased. The expression of E-cadherin was increased, but the expression of N-cadherin, vimentin and snail were decreased. Increased expression of lncRNASNHG7 in cancer tissues was significantly related to tumor size, metastasis and stage. CONCLUSIONS: This study showed that lncRNA -SNHG7 is overexpressed in bladder cancer tissues and cells. Downregulation of lncRNA-SNHG7 can inhibit the proliferation of bladder cancer cells and promote apoptosis, as well as inhibit cell invasion, which provides a potential molecular target for future tumor targeted therapy.

Asunto(s)

Apoptosis/genética; Proliferación Celular/genética; Invasividad Neoplásica/genética; ARN Largo no Codificante/genética; Neoplasias de la Vejiga Urinaria/genética; Cadherinas/genética; Línea Celular Tumoral; Movimiento Celular/genética; Regulación hacia Abajo/genética; Transición Epitelial-Mesenquimal/genética; Regulación Neoplásica de la Expresión Génica/genética; Humanos; Interferencia de ARN/fisiología; ARN Interferente Pequeño/genética; Regulación hacia Arriba/genética; Vejiga Urinaria/patología; Neoplasias de la Vejiga Urinaria/patología

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Vejiga Urinaria / Apoptosis / Proliferación Celular / ARN Largo no Codificante / Invasividad Neoplásica Tipo de estudio: Guideline Límite: Humans Idioma: En Revista: J BUON Asunto de la revista: NEOPLASIAS Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Chipre

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