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Interleukin-35 Suppresses the Antitumor Activity of T Cells in Patients with Non-Small Cell Lung Cancer.
Wang, Hong-Min; Zhang, Xiao-Hong; Feng, Ming-Ming; Qiao, Yan-Jun; Ye, Li-Qun; Chen, Jing; Fan, Fei-Fei; Guo, Lin-Lin.
Afiliación
  • Wang HM; Department of Respiratory Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
  • Zhang XH; Department of Respiratory Medicine, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, China.
  • Feng MM; Department of Respiratory Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
  • Qiao YJ; Department of Respiratory Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
  • Ye LQ; Department of Respiratory Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
  • Chen J; Department of Respiratory Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
  • Fan FF; Department of Respiratory Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
  • Guo LL; Department of Respiratory Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Cell Physiol Biochem ; 47(6): 2407-2419, 2018.
Article en En | MEDLINE | ID: mdl-29991058
BACKGROUND/AIMS: Interleukin (IL)-35 has immunosuppressive functions in autoimmune diseases, infectious diseases, and certain cancers. However, few studies have focused on its immunoregulatory activity in non-small cell lung cancer (NSCLC). Thus, we investigated the role of IL-35 in the pathogenesis of this disease. METHODS: A total of 66 NSCLC patients and 21 healthy individuals were enrolled. IL-35 expression in peripheral blood and bronchoalveolar lavage fluid (BALF) was measured. The modulatory functions of IL-35 on purified CD4+ and CD8+ T cells from NSCLC patients were investigated in direct and indirect coculture systems with NSCLC cell lines. RESULTS: IL-35 expression was significantly increased in BALF from the tumor site, but not in the peripheral blood of NSCLC patients. IL-35 did not affect the bioactivity including proliferation, cytokine production, cell cycle, and cellular invasion of NSCLC cells. It suppressed responses from type 1 T helper (Th1) and Th17 cells but elevated the regulatory T cell response in cultured CD4+ T cells from NSCLC patients, and reduced cytokine-mediated CD4+ T cells cytotoxicity to NSCLC cells. Moreover, IL-35 also inhibited cytotoxic gene expression in CD8+ T cells from NSCLC, reducing their cytolytic and noncytolytic functions. CONCLUSION: The results of this study suggest that IL-35 contributes to the dysfunction/exhaustion of T cells and limited antitumor immune responses in NSCLC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T CD4-Positivos / Regulación Neoplásica de la Expresión Génica / Interleucinas / Carcinoma de Pulmón de Células no Pequeñas / Linfocitos T CD8-positivos / Neoplasias Pulmonares / Proteínas de Neoplasias Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Cell Physiol Biochem Asunto de la revista: BIOQUIMICA / FARMACOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T CD4-Positivos / Regulación Neoplásica de la Expresión Génica / Interleucinas / Carcinoma de Pulmón de Células no Pequeñas / Linfocitos T CD8-positivos / Neoplasias Pulmonares / Proteínas de Neoplasias Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Cell Physiol Biochem Asunto de la revista: BIOQUIMICA / FARMACOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania