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Simultaneous quantification of Gemcitabine and Irinotecan hydrochloride in rat plasma by using high performance liquid chromatography-diode array detector.
Malatesta, L; Cosco, D; Paolino, D; Cilurzo, F; Costa, N; Di Tullio, A; Fresta, M; Celia, C; Di Marzio, L; Locatelli, M.
Afiliación
  • Malatesta L; University of Chieti - Pescara "G. d'Annunzio", Department of Pharmacy, via dei Vestini 31, 66100 Chieti, Italy.
  • Cosco D; University of Catanzaro "Magna Graecia", Department of Health Sciences, Viale "S. Venuta" s.n.c., 88100 Catanzaro, Italy.
  • Paolino D; University of Catanzaro "Magna Graecia", Department of Experimental and Clinical Medicine, Viale "S. Venuta" s.n.c., 88100 Catanzaro, Italy; University of Catanzaro "Magna Græcia", Inter-Regional Research Center for Food Safety & Health, Viale "S. Venuta" s.n.c., 88100 Catanzaro, Italy.
  • Cilurzo F; University of Chieti - Pescara "G. d'Annunzio", Department of Pharmacy, via dei Vestini 31, 66100 Chieti, Italy.
  • Costa N; University of Catanzaro "Magna Graecia", Department of Health Sciences, Viale "S. Venuta" s.n.c., 88100 Catanzaro, Italy.
  • Di Tullio A; University of Chieti - Pescara "G. d'Annunzio", Department of Pharmacy, via dei Vestini 31, 66100 Chieti, Italy.
  • Fresta M; University of Catanzaro "Magna Graecia", Department of Health Sciences, Viale "S. Venuta" s.n.c., 88100 Catanzaro, Italy.
  • Celia C; University of Chieti - Pescara "G. d'Annunzio", Department of Pharmacy, via dei Vestini 31, 66100 Chieti, Italy; Houston Methodist Research Institute, Department of Nanomedicine, 6670 Bertner Ave, Houston, TX 77030, USA.
  • Di Marzio L; University of Chieti - Pescara "G. d'Annunzio", Department of Pharmacy, via dei Vestini 31, 66100 Chieti, Italy.
  • Locatelli M; University of Chieti - Pescara "G. d'Annunzio", Department of Pharmacy, via dei Vestini 31, 66100 Chieti, Italy; Interuniversity Consortium of Structural and Systems Biology, Viale Medaglie d'Oro 305, 00136 Roma, Italy. Electronic address: m.locatelli@unich.it.
J Pharm Biomed Anal ; 159: 192-199, 2018 Sep 10.
Article en En | MEDLINE | ID: mdl-29990886
In this manuscript we aimed at the simultaneous separation and quantification of Gemcitabine and Irinotecan hydrochloride (injected both as single components and in combination) from Sprague Dawley rat plasma by using a validated method obtained through the use of a High Performance Liquid Chromatography (HPLC)-diode array detector (DAD). Gemcitabine and Irinotecan hydrochloride were detected and quantified using a Zorbax Extend C-18 column (250 mm × 4.6 mm; 5 µm particle size) in gradient elution mode. The chromatographic analyses were carried out in 15 min. The analytical mode was calibrated and validated in the concentration range from 0.1 to 18 µg/mL both for Gemcitabine and Irinotecan hydrochloride. Sprague Dawley rat plasma was used to perform the analysis. 3-methylxanthine was the internal standard. The weighted-matrix matched standard curves of Gemcitabine and Irinotecan hydrochloride showed a good linearity up to 18 µg/mL. Parallelism tests were also performed to evaluate whether the over-range samples could be analyzed after dilution without affecting the analytical performance. The intra- and inter-day precision (RSD%) values of Gemcitabine and Irinotecan hydrochloride were ≤7.14% and ≤11.5%, respectively. The intra- and inter-day trueness (Bias%) values were in the range from -11.5% to 1.70% for both drugs. The analytical mode performance was further tested after collecting Sprague Dawley rat plasma following a single-dose administration of chemotherapeutics or their association. The validated HPLC-DAD method allowed the simultaneous quantification of Gemcitabine and Irinotecan hydrochloride in the rat plasma, besides the evaluation of the pharmacokinetic parameters and drug delivery.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Camptotecina / Técnicas de Química Analítica / Desoxicitidina / Antimetabolitos Antineoplásicos / Antineoplásicos Fitogénicos Límite: Animals Idioma: En Revista: J Pharm Biomed Anal Año: 2018 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Camptotecina / Técnicas de Química Analítica / Desoxicitidina / Antimetabolitos Antineoplásicos / Antineoplásicos Fitogénicos Límite: Animals Idioma: En Revista: J Pharm Biomed Anal Año: 2018 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido