Heat shock factor 1 regulates heat shock proteins and immune-related genes in Penaeus monodon under thermal stress.

Sornchuer, Phornphan; Junprung, Wisarut; Yingsunthonwattana, Warumporn; Tassanakajon, Anchalee

Sornchuer, Phornphan; Junprung, Wisarut; Yingsunthonwattana, Warumporn; Tassanakajon, Anchalee.

Afiliación

Sornchuer P; Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Junprung W; Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Yingsunthonwattana W; Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Tassanakajon A; Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand; Omics Science and Bioinformatics Center, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand. Electronic address:

Dev Comp Immunol ; 88: 19-27, 2018 11.

Article en En | MEDLINE | ID: mdl-29986835

RESUMEN

Heat shock factors (HSFs) participate in the response to environmental stressors and regulate heat shock protein (Hsp) expression. This study describes the molecular characterization and expression of PmHSF1 in black tiger shrimp Penaeus monodon under heat stress. PmHSF1 expression was detected in several shrimp tissues: the highest in the lymphoid organ and the lowest in the eyestalk. Significant up-regulation of PmHSF1 expression was observed in hemocytes (pâ¯<â¯0.05) following thermal stress. The expression of several PmHsps was rapidly induced following heat stress. Endogenous PmHSF1 protein was expressed in all three types of shrimp hemocyte and strongly induced under heat stress. The suppression of PmHSF1 expression by dsRNA-mediated gene silencing altered the expression of PmHsps, several antimicrobial genes, genes involved in the melanization process, and an antioxidant gene (PmSOD). PmHSF1 plays an important role in the thermal stress response, regulating the expression of Hsps and immune-related genes in P. monodon.

Asunto(s)

Proteínas de Artrópodos/metabolismo; Factores de Transcripción del Choque Térmico/metabolismo; Respuesta al Choque Térmico/inmunología; Inmunidad Humoral/genética; Penaeidae/inmunología; Animales; Proteínas de Artrópodos/genética; ADN Complementario/genética; Perfilación de la Expresión Génica; Técnicas de Silenciamiento del Gen; Silenciador del Gen/inmunología; Factores de Transcripción del Choque Térmico/genética; Proteínas de Choque Térmico/genética; Hemocitos/inmunología; Hemocitos/metabolismo; Penaeidae/genética; ARN Bicatenario/metabolismo; Regulación hacia Arriba

Palabras clave

Heat shock factor; Heat shock protein; Penaeus monodon; Shrimp immunity; Thermal stress

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Respuesta al Choque Térmico / Penaeidae / Inmunidad Humoral / Proteínas de Artrópodos / Factores de Transcripción del Choque Térmico Límite: Animals Idioma: En Revista: Dev Comp Immunol Año: 2018 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Estados Unidos

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