The temperate B. subtilis phage phi 105 genome contains at least two distinct regions encoding superinfection immunity.
Mol Gen Genet
; 200(3): 490-2, 1985.
Article
en En
| MEDLINE
| ID: mdl-2995768
Two different PstI fragments of temperate phage phi 105 DNA are shown to confer superinfection immunity upon Bacillus subtilis when inserted into the multicopy cloning vector pE194 cop-6. The 2.3 kb PstI fragment I is located almost entirely within EcoRI fragment F and encompasses a region previously known to encode a repressor. The other fragment, PstI-E (4.3 kb) maps inside the EcoRI-B fragment, and allows an explanation of the clear-plaque phenotype of the deletion mutant phi 105DII:6c. The two regions can be distinguished functionally, since only the PstI fragment I product interacts with a specific phi 105 promoter-operator site.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacillus subtilis
/
Bacteriófagos
/
Genes Virales
Idioma:
En
Revista:
Mol Gen Genet
Año:
1985
Tipo del documento:
Article
Pais de publicación:
Alemania