Cryo-electron tomography of SYCP3 fibers under native conditions.
Methods Cell Biol
; 145: 347-371, 2018.
Article
en En
| MEDLINE
| ID: mdl-29957214
The synaptonemal complex (SC) forms during the early stages of meiotic prophase I, when it mediates the pairing of homologous chromosomes. Despite the crucial role of the SC in chromosome synapsis and genetic recombination, the molecular details of its function are still unclear. High-resolution information on the structure of SC proteins would be very valuable to elucidate the molecular basis of their function in meiosis. Here we show how cryo-electron tomography and subtomographic averaging can be usefully applied to provide insights into the structure of the helical SYCP3 protein in its filamentous state. The establishment of such method should prove of use for structural studies of other SC proteins, such as SYCP1 and the TEX12-SYCE2 complex, which can form physiologically relevant filamentous assemblies, and ultimately for the structural analysis of the SC.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Nucleares
/
Tomografía con Microscopio Electrónico
Límite:
Humans
Idioma:
En
Revista:
Methods Cell Biol
Año:
2018
Tipo del documento:
Article
País de afiliación:
Reino Unido
Pais de publicación:
Estados Unidos