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Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation.
Shin, Dong Jin; Trick, Alexander Y; Hsieh, Yu-Hsiang; Thomas, David L; Wang, Tza-Huei.
Afiliación
  • Shin DJ; Department of Mechanical Engineering, Whiting School of Engineering, The Johns Hopkins University, Baltimore, MD, United States.
  • Trick AY; Department of Biomedical Engineering, Whiting School of Engineering, The Johns Hopkins University, Baltimore, MD, United States.
  • Hsieh YH; Department of Emergency Medicine, School of Medicine, The Johns Hopkins University, Baltimore, MD, United States.
  • Thomas DL; Department of Medicine, School of Medicine, The Johns Hopkins University, Baltimore, MD, United States.
  • Wang TH; Infectious Disease Center for Viral Hepatitis, School of Medicine, The Johns Hopkins University, Baltimore, MD, United States.
Sci Rep ; 8(1): 9793, 2018 06 28.
Article en En | MEDLINE | ID: mdl-29955160
Gold standard quantitative nucleic acid tests for diagnosis of viral diseases are currently limited to implementation in laboratories outside of the clinic. An instrument for conducting nucleic acid testing at the point-of-care (POC) that is easily operable by the clinician would reduce the required number of visits to the clinic and improve patient retention for proper treatment. Here we present a droplet magnetofluidic (DM) platform, which leverages functionalized magnetic particles to miniaturize and automate laboratory assays for use in the clinic at the POC. Our novel thermoformed disposable cartridge coupled to a portable multiaxial magnetofluidic instrument enables real-time PCR assays for quantitative and sensitive detection of nucleic acids from crude biosamples. Instead of laborious benchtop sample purification techniques followed by elution and spiking into PCR buffer, the user simply injects the biosample of interest into a cartridge with magnetic particles and loads the cartridge into the instrument. We demonstrate the utility of our platform with hepatitis C virus (HCV) RNA viral load quantitation from blood serum in approximately 1 hour. Clinical serum samples (n = 18) were directly processed on cartridges with no false positives and a limit of detection of 45 IU per 10 µl sample injection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hepacivirus / Sistemas de Atención de Punto / Carga Viral / Microfluídica / Magnetismo Límite: Humans Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hepacivirus / Sistemas de Atención de Punto / Carga Viral / Microfluídica / Magnetismo Límite: Humans Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido