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A new dual-targeting real-time RT-PCR assay for hepatitis D virus RNA detection.
Wang, Yan; Glenn, Jeffrey S; Winters, Mark A; Shen, Li-Ping; Choong, Ingrid; Shi, Ya-Lun; Bi, Sheng-Li; Ma, Li-Ying; Zeng, Hui; Zhang, Fu-Jie.
Afiliación
  • Wang Y; State Key Laboratory of Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing, P.R. China; Clinical and Research Ce
  • Glenn JS; Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA.
  • Winters MA; Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA.
  • Shen LP; National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
  • Choong I; Eiger BioPharmaceuticals, Inc. Palo Alto, California, USA.
  • Shi YL; Beijing Anapure BioScientific Co. Ltd, Beijing, China.
  • Bi SL; National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
  • Ma LY; State Key Laboratory of Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing, P.R. China.
  • Zeng H; Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, China.
  • Zhang FJ; Clinical and Research Center of Infectious Diseases, Beijing Ditan Hospital, Clinical Center for HIV/AIDS, Capital Medical University, Beijing, P.R. China. Electronic address: treatment@chinaaids.cn.
Diagn Microbiol Infect Dis ; 92(2): 112-117, 2018 Oct.
Article en En | MEDLINE | ID: mdl-29941366
In this study, a real-time reverse transcription-polymerase chain reaction (real time RT-PCR) assay targeting 2 genetic segments was established to detect HDV RNA. Utilizing the World Health Organization International Standard for Hepatitis D Virus RNA, the lower limit of detection was 575 IU/mL, and the linearity of quantification ranged from 575,000 IU/mL to 575 IU/mL. 384 HBsAg-positive samples collected from China were tested by this method and HDV antibody detection. Eleven samples were positive for anti-HDV IgG which may persist after HDV resolution, 6 samples were HDV RNA positive, and 5 samples were positive for anti-HDV IgM. This assay showed more sensitivity than the detection of anti-HDV IgM. These data demonstrate that the real-time RT-PCR assay for HDV RNA could be implemented in the clinical detection of HDV infection in chronic HBV-infected patients in China.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hepatitis D / Virus de la Hepatitis Delta / Anticuerpos Antihepatitis / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa Tipo de estudio: Diagnostic_studies Límite: Adult / Female / Humans / Male País/Región como asunto: Asia Idioma: En Revista: Diagn Microbiol Infect Dis Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hepatitis D / Virus de la Hepatitis Delta / Anticuerpos Antihepatitis / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa Tipo de estudio: Diagnostic_studies Límite: Adult / Female / Humans / Male País/Región como asunto: Asia Idioma: En Revista: Diagn Microbiol Infect Dis Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos