Identification of a receptor for atrial natriuretic factor in rabbit aorta membranes by affinity cross-linking.
J Biol Chem
; 260(20): 10889-92, 1985 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-2993293
Binding sites in rabbit aorta membranes for atrial natriuretic factor (ANF) have been specifically and covalently labeled by two methods. In the first, the photoreactive analog of ANF, 125I-azidobenzoyl-ANF, was synthesized and used to photoaffinity label ANF receptors. In the second, 125I-ANF was covalently attached to its binding site by treatment of the 125I-ANF-receptor complex with bifunctional cross-linking agents. Analysis of the labeled proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography revealed that by both methods the same three protein bands were labeled. These bands had apparent molecular masses of 60,000, 70,000, and 120,000 daltons. With the photoaffinity label, half-maximal inhibition of labeling of each of these bands was achieved when approximately 200 pM of unlabeled ANF was included in the binding assay. These results suggest that these three different polypeptides are specific components of ANF receptors in rabbit aorta membranes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Aorta
/
Receptores de Superficie Celular
/
Proteínas Musculares
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Biol Chem
Año:
1985
Tipo del documento:
Article
Pais de publicación:
Estados Unidos