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Increasing the stability of Lumbricus terrestris erythrocruorin via poly(acrylic acid) conjugation.
Spivack, Kyle; Tucker, Matthew; Zimmerman, Devon; Nicholas, Matthew; Abdulmalik, Osheiza; Comolli, Noelle; Elmer, Jacob.
Afiliación
  • Spivack K; a Department of Chemical Engineering , Villanova University , Villanova , PA , USA.
  • Tucker M; a Department of Chemical Engineering , Villanova University , Villanova , PA , USA.
  • Zimmerman D; a Department of Chemical Engineering , Villanova University , Villanova , PA , USA.
  • Nicholas M; a Department of Chemical Engineering , Villanova University , Villanova , PA , USA.
  • Abdulmalik O; b Division of Hematology , The Children's Hospital of Philadelphia , Philadelphia , PA , USA.
  • Comolli N; a Department of Chemical Engineering , Villanova University , Villanova , PA , USA.
  • Elmer J; a Department of Chemical Engineering , Villanova University , Villanova , PA , USA.
Artif Cells Nanomed Biotechnol ; 46(sup2): 1137-1144, 2018.
Article en En | MEDLINE | ID: mdl-29916733
Since donated red blood cells must be constantly refrigerated, they are often unavailable in remote areas and battlefields. The goal of this study was to synthesize a highly stable blood substitute that does not require refrigeration. Specifically, the extracellular haemoglobin (a.k.a. erythrocruorin, Ec) of the earthworm Lumbricus terrestris erythrocruororin (LtEc) was cross-linked with poly(acrylic acid) (PAA) and ethylene diamine (EDA). PAGE analysis of the LtEc nanoparticles reveals cross-linking between subunits, while dynamic light scattering and scanning electron microscopy show that cross-linking significantly increases the size of the LtEc nanoparticles (164 ± 13.9 nm). Cross-linking also significantly increased the thermal stability of the LtEc nanoparticles by 10 °C (Tm = 72 ± 0.84 °C) relative to native LtEc (Tm = 62 ± 0.6 °C). In addition, while native LtEc rapidly dissociates at pH 9, the LtEc nanoparticles resist subunit dissociation up to pH 10. The oxygen affinity of the LtEc nanoparticles (P50 = 6.85 ± 0.13 mm Hg) is much higher than native LtEc (P50 = 26.67 ± 0.4 mm Hg), but the cooperativity (n = 2.43 ± 0.12) is not affected. Altogether, these results show that cross-linking LtEc with PAA and EDA provides a potential blood substitute with increased stability and oxygen affinity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligoquetos / Resinas Acrílicas / Eritrocruorinas Límite: Animals Idioma: En Revista: Artif Cells Nanomed Biotechnol Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligoquetos / Resinas Acrílicas / Eritrocruorinas Límite: Animals Idioma: En Revista: Artif Cells Nanomed Biotechnol Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido