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Mesenchymal stem cells in osteotomy repair after tibial tuberosity advancement in dogs with cranial cruciate ligament injury.
Rocha Dos Santos, Clarissa; da Rocha Filgueiras, Richard; Furtado Malard, Patrícia; Rodrigues da Cunha Barreto-Vianna, Andre; Nogueira, Kaique; da Silva Leite, Carolina; Maurício Mendes de Lima, Eduardo.
Afiliación
  • Rocha Dos Santos C; Faculty of Agronomy and Veterinary Medicine, University of Brasilia, Brasília, DF, Brazil.
  • da Rocha Filgueiras R; Orthos Ortopedia e Neurocirurgia Veterinária, Brasília, DF, Brazil.
  • Furtado Malard P; BIO-Biotecnologia em Reprodução Animal, Brasília, DF, Brazil.
  • Rodrigues da Cunha Barreto-Vianna A; Faculty of Veterinary Medicine, Federal University of Lavras, Lavras, MG, Brazil.
  • Nogueira K; Faculty of Agronomy and Veterinary Medicine, University of Brasilia, Brasília, DF, Brazil.
  • da Silva Leite C; Faculty of Agronomy and Veterinary Medicine, University of Brasilia, Brasília, DF, Brazil.
  • Maurício Mendes de Lima E; Faculty of Agronomy and Veterinary Medicine, University of Brasilia, Brasília, DF, Brazil. limaemm@unb.br.
J Exp Orthop ; 5(1): 17, 2018 Jun 14.
Article en En | MEDLINE | ID: mdl-29900517
BACKGROUND: The cranial cruciate ligament rupture (CCLR) is the most commonly encountered orthopedic condition in dogs. Among the various techniques to treat this condition, tibial tuberosity advancement (TTA) has been used to obtain rapid recovery of the affected knee. The objective of this study was to evaluate the viability of the use of mesenchymal stem cells (MSC) implanted in the osteotomy site obtained by TTA in nine dogs diagnosed with CCLR. METHODS: The MSC were isolated from the adipose tissue of the dogs and cultured for eight days, the animals were divided into two groups. Animals from the treated group (GT) received cell transport medium containing about 1.5 millions MSC, and the animals from the control group (GC) received only the cell transport medium. The study was performed in a double-blind manner using radiographs acquired on days 15, 30, 60 and 120 after the procedure. Evaluations of the density of the trabecular bone were performed using image analysis software. The results were subjected to descriptive statistical analysis, followed by the normality test, Chi-square test, Mann-Whitney test and Tukey's multiple comparison test for p ≤ 0.05. RESULTS: After 30 days of the procedure, the animals of the GT presented an ossification mean 36.45% greater (p ≤ 0.033) than the GC, and there were no statistical differences for the other periods. CONCLUSIONS: Despite the total bone ossification within the expected period, there was no minimization of the estimated recovery time with the application of MSC, and inflammatory factors should be considered for reassessment of the therapeutic intervention time.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Clinical_trials Idioma: En Revista: J Exp Orthop Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Clinical_trials Idioma: En Revista: J Exp Orthop Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania