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Acute depletion of diacylglycerol from the cis-Golgi affects localized nuclear envelope morphology during mitosis.
Chung, Gary Hong Chun; Domart, Marie-Charlotte; Peddie, Christopher; Mantell, Judith; Mclaverty, Kieran; Arabiotorre, Angela; Hodgson, Lorna; Byrne, Richard D; Verkade, Paul; Arkill, Kenton; Collinson, Lucy M; Larijani, Banafshé.
Afiliación
  • Chung GHC; Cell Biophysics Laboratory, Ikerbasque Basque Foundation for Science, Research Centre for Experimental Marine Biology and Biotechnology (PiE) and Biofísika Institute (UPV/EHU, CSIC), University of the Basque Country, Barrio Sarriena s/n 48940, Leioa, Spain.
  • Domart MC; Electron Microscopy Science Technology Platform, Francis Crick Institute, London, United Kingdom.
  • Peddie C; Electron Microscopy Science Technology Platform, Francis Crick Institute, London, United Kingdom.
  • Mantell J; School of Biochemistry, Faculty of Biomedical Sciences, University of Bristol, Bristol, United Kingdom.
  • Mclaverty K; Cell Biophysics Laboratory, Ikerbasque Basque Foundation for Science, Research Centre for Experimental Marine Biology and Biotechnology (PiE) and Biofísika Institute (UPV/EHU, CSIC), University of the Basque Country, Barrio Sarriena s/n 48940, Leioa, Spain.
  • Arabiotorre A; School of Biochemistry, Faculty of Biomedical Sciences, University of Bristol, Bristol, United Kingdom.
  • Hodgson L; Cell Biophysics Laboratory, Ikerbasque Basque Foundation for Science, Research Centre for Experimental Marine Biology and Biotechnology (PiE) and Biofísika Institute (UPV/EHU, CSIC), University of the Basque Country, Barrio Sarriena s/n 48940, Leioa, Spain.
  • Byrne RD; School of Biochemistry, Faculty of Biomedical Sciences, University of Bristol, Bristol, United Kingdom.
  • Verkade P; Cell Biophysics Laboratory, Ikerbasque Basque Foundation for Science, Research Centre for Experimental Marine Biology and Biotechnology (PiE) and Biofísika Institute (UPV/EHU, CSIC), University of the Basque Country, Barrio Sarriena s/n 48940, Leioa, Spain.
  • Arkill K; School of Biochemistry, Faculty of Biomedical Sciences, University of Bristol, Bristol, United Kingdom.
  • Collinson LM; Wolfson Bioimaging Facility, University of Bristol, Bristol, United Kingdom.
  • Larijani B; Cell Biophysics Laboratory, Ikerbasque Basque Foundation for Science, Research Centre for Experimental Marine Biology and Biotechnology (PiE) and Biofísika Institute (UPV/EHU, CSIC), University of the Basque Country, Barrio Sarriena s/n 48940, Leioa, Spain Kenton.Arkill@nottingham.ac.uk lucy.collins
J Lipid Res ; 59(8): 1402-1413, 2018 08.
Article en En | MEDLINE | ID: mdl-29895700
Dysregulation of nuclear envelope (NE) assembly results in various cancers; for example, renal and some lung carcinomas ensue due to NE malformation. The NE is a dynamic membrane compartment and its completion during mitosis is a highly regulated process, but the detailed mechanism still remains incompletely understood. Previous studies have found that isolated diacylglycerol (DAG)-containing vesicles are essential for completing the fusion of the NE in nonsomatic cells. We investigated the impact of DAG depletion from the cis-Golgi in mammalian cells on NE reassembly. Using advanced electron microscopy, we observed an enriched DAG population of vesicles at the vicinity of the NE gaps of telophase mammalian cells. We applied a mini singlet oxygen generator-C1-domain tag that localized DAG-enriched vesicles at the perinuclear region, which suggested the existence of NE fusogenic vesicles. We quantified the impact of Golgi-DAG depletion by measuring the in situ NE rim curvature of the reforming NE. The rim curvature in these cells was significantly reduced compared with controls, which indicated a localized defect in NE morphology. Our novel results demonstrate the significance of the role of DAG from the cis-Golgi for the regulation of NE assembly.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diglicéridos / Aparato de Golgi / Mitosis / Membrana Nuclear Límite: Humans Idioma: En Revista: J Lipid Res Año: 2018 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diglicéridos / Aparato de Golgi / Mitosis / Membrana Nuclear Límite: Humans Idioma: En Revista: J Lipid Res Año: 2018 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos