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Effects of sampling effort on biodiversity patterns estimated from environmental DNA metabarcoding surveys.
Grey, Erin K; Bernatchez, Louis; Cassey, Phillip; Deiner, Kristy; Deveney, Marty; Howland, Kimberly L; Lacoursière-Roussel, Anaïs; Leong, Sandric Chee Yew; Li, Yiyuan; Olds, Brett; Pfrender, Michael E; Prowse, Thomas A A; Renshaw, Mark A; Lodge, David M.
Afiliación
  • Grey EK; Division of Science, Mathematics and Technology, Governors State University, 1 University Parkway, University Park, Illinois, 60484, USA. egrey@govst.edu.
  • Bernatchez L; Insitut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, 1030 Avenue de la Médecine, Québec, G1V 0A6, Canada.
  • Cassey P; School of Biological Sciences, University of Adelaide, Adelaide, SA, 5005, Australia.
  • Deiner K; Department of Ecology and Evolutionary Biology, Cornell University, 200 Rice Hall, Ithaca, NY, 14853, USA.
  • Deveney M; South Australian Aquatic Sciences Centre, 2 Hamra Avenue, West Beach, SA, 5024, Australia.
  • Howland KL; Fisheries and Oceans Canada, 501 University Crescent, Winnipeg, Manitoba, R3T 2N6, Canada.
  • Lacoursière-Roussel A; Insitut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, 1030 Avenue de la Médecine, Québec, G1V 0A6, Canada.
  • Leong SCY; Tropical Marine Science Institute, National University of Singapore, 18 Kent Ridge Road, S2S Building, Singapore, 119227, Singapore.
  • Li Y; Department of Biological Sciences, University of Notre Dame, 109b Galvin Life Science Center, Notre Dame, IN, 46556, USA.
  • Olds B; Oceanic Institute, Hawaii Pacific University, 41-202 Kalanianaole Highway, Waimanalo, HI, 96795, USA.
  • Pfrender ME; Department of Biological Sciences, University of Notre Dame, 109b Galvin Life Science Center, Notre Dame, IN, 46556, USA.
  • Prowse TAA; Environmental Change Initiative, University of Notre Dame, 1400 East Angela Boulevard, Unit 117, South Bend, IN, 46617, USA.
  • Renshaw MA; School of Biological Sciences, University of Adelaide, Adelaide, SA, 5005, Australia.
  • Lodge DM; School of Mathematical Sciences, University of Adelaide, Adelaide, SA, 5005, Australia.
Sci Rep ; 8(1): 8843, 2018 06 11.
Article en En | MEDLINE | ID: mdl-29891968
Environmental DNA (eDNA) metabarcoding can greatly enhance our understanding of global biodiversity and our ability to detect rare or cryptic species. However, sampling effort must be considered when interpreting results from these surveys. We explored how sampling effort influenced biodiversity patterns and nonindigenous species (NIS) detection in an eDNA metabarcoding survey of four commercial ports. Overall, we captured sequences from 18 metazoan phyla with minimal differences in taxonomic coverage between 18 S and COI primer sets. While community dissimilarity patterns were consistent across primers and sampling effort, richness patterns were not, suggesting that richness estimates are extremely sensitive to primer choice and sampling effort. The survey detected 64 potential NIS, with COI identifying more known NIS from port checklists but 18 S identifying more operational taxonomic units shared between three or more ports that represent un-recorded potential NIS. Overall, we conclude that eDNA metabarcoding surveys can reveal global similarity patterns among ports across a broad array of taxa and can also detect potential NIS in these key habitats. However, richness estimates and species assignments require caution. Based on results of this study, we make several recommendations for port eDNA sampling design and suggest several areas for future research.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Biodiversidad / Ambiente / Metagenómica / Código de Barras del ADN Taxonómico Límite: Animals Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Biodiversidad / Ambiente / Metagenómica / Código de Barras del ADN Taxonómico Límite: Animals Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido