Construction of a human X-chromosome-enriched phage library which facilitates analysis of specific loci.

Kunkel, L M; Lalande, M; Monaco, A P; Flint, A; Middlesworth, W; Latt, S A

Kunkel, L M; Lalande, M; Monaco, A P; Flint, A; Middlesworth, W; Latt, S A.

Gene ; 33(3): 251-8, 1985.

Article en En | MEDLINE | ID: mdl-2989089

RESUMEN

A human X-chromosome-enriched MboI-partial-digest recombinant library in phage lambda Charon30 has been constructed. Twelve out of the thirteen X-chromosome DNA sequences that were tested were present in the library. Most regions were covered in overlapping phage inserts; mean insert size was 13.7 kb. One phage from the library allowed detection of a 225-bp insertion of DNA into a region near the Duchenne muscular dystrophy (DMD) locus. Another recombinant phage represents an expansion of a region which exhibits extensive and varying homology with other human chromosomes, including the Y, as well as with rodent DNA. The present library should have widespread use for examining DNA sequences on the human X chromosome.

Asunto(s)

Mapeo Cromosómico; Desoxirribonucleasas de Localización Especificada Tipo II; Ligamiento Genético; Distrofias Musculares/genética; Cromosoma X; Bacteriófago lambda/genética; Secuencia de Bases; Línea Celular; Clonación Molecular; ADN/genética; Enzimas de Restricción del ADN; ADN Recombinante; ADN Viral/genética; Femenino; Humanos; Hibridación de Ácido Nucleico

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromosoma X / Desoxirribonucleasas de Localización Especificada Tipo II / Mapeo Cromosómico / Ligamiento Genético / Distrofias Musculares Límite: Female / Humans Idioma: En Revista: Gene Año: 1985 Tipo del documento: Article Pais de publicación: Países Bajos

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