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Skin Microbiota in Obese Women at Risk for Surgical Site Infection After Cesarean Delivery.
Rood, Kara M; Buhimschi, Irina A; Jurcisek, Joseph A; Summerfield, Taryn L; Zhao, Guomao; Ackerman, William E; Wang, Weiwei; Rumpf, R Wolfgang; Thung, Stephen F; Bakaletz, Lauren O; Buhimschi, Catalin S.
Afiliación
  • Rood KM; Department of Obstetrics and Gynecology, The Ohio State University College of Medicine, Columbus, Ohio, 43210, USA. kara.rood@osumc.edu.
  • Buhimschi IA; Center for Perinatal Research, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, 43215, USA.
  • Jurcisek JA; Department of Pediatrics, The Ohio State University College of Medicine, Columbus, Ohio, 43210, USA.
  • Summerfield TL; Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, 43215, USA.
  • Zhao G; Department of Obstetrics and Gynecology, The Ohio State University College of Medicine, Columbus, Ohio, 43210, USA.
  • Ackerman WE; Center for Perinatal Research, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, 43215, USA.
  • Wang W; Department of Obstetrics and Gynecology, The Ohio State University College of Medicine, Columbus, Ohio, 43210, USA.
  • Rumpf RW; Center for Perinatal Research, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, 43215, USA.
  • Thung SF; Battelle Center for Mathematical Medicine, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, 43215, USA.
  • Bakaletz LO; Department of Obstetrics and Gynecology, The Ohio State University College of Medicine, Columbus, Ohio, 43210, USA.
  • Buhimschi CS; Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, 43215, USA.
Sci Rep ; 8(1): 8756, 2018 06 08.
Article en En | MEDLINE | ID: mdl-29884793
The obesity pandemic in the obstetrical population plus increased frequency of Cesarean delivery (CD) has increased vulnerability to surgical site infection (SSI). Here we characterized the microbiome at the site of skin incision before and after CD. Skin and relevant surgical sites were sampled before and after surgical antisepsis from obese (n = 31) and non-obese (n = 27) pregnant women. We quantified bacterial biomass by qPCR, microbial community composition by 16sRNA sequencing, assigned operational taxonomic units, and stained skin biopsies from incision for bacteria and biofilms. In obese women, incision site harbors significantly higher bacterial biomass of lower diversity. Phylum Firmicutes predominated over Actinobacteria, with phylotypes Clostridales and Bacteroidales over commensal Staphylococcus and Propionbacterium spp. Skin dysbiosis increased post-surgical prep and at end of surgery. Biofilms were identified post-prep in the majority (73%) of skin biopsies. At end of surgery, incision had significant gains in bacterial DNA and diversity, and obese women shared more genera with vagina and surgeon's glove in CD. Our findings suggest microbiota at incision differs between obese and non-obese pregnant women, and changes throughout CD. An interaction between vaginal and cutaneous dysbiosis at the incision site may explain the a priori increased risk for SSI among obese pregnant women.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piel / Infección de la Herida Quirúrgica / Bacterias / Cesárea / Obesidad Tipo de estudio: Etiology_studies / Prognostic_studies / Risk_factors_studies Límite: Female / Humans / Pregnancy Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piel / Infección de la Herida Quirúrgica / Bacterias / Cesárea / Obesidad Tipo de estudio: Etiology_studies / Prognostic_studies / Risk_factors_studies Límite: Female / Humans / Pregnancy Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido