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Expansion of transplanted islets in mice by co-transplantation with adipose tissue-derived mesenchymal stem cells.
Tanaka, Tomoko; Kojima, Daibo; Mera, Toshiyuki; Matsumoto, Masahito; Yasunami, Yohichi; Yanase, Toshihiko.
Afiliación
  • Tanaka T; Department of Bioregulatory Science of Life-related Diseases, Faculty of Medicine Fukuoka University, Fukuoka, Japan.
  • Kojima D; Department of Regenerative Medicine and Transplantation, Faculty of Medicine Fukuoka University, Fukuoka, Japan.
  • Mera T; Department of Gastroenterological Surgery, Faculty of Medicine Fukuoka University, Fukuoka, Japan.
  • Matsumoto M; Department of Regenerative Medicine and Transplantation, Faculty of Medicine Fukuoka University, Fukuoka, Japan.
  • Yasunami Y; Department of Gastroenterological Surgery, Faculty of Medicine Fukuoka University, Fukuoka, Japan.
  • Yanase T; Center of Genomic Medicine, Saitama Medical University, Saitama, Japan.
Heliyon ; 4(5): e00632, 2018 May.
Article en En | MEDLINE | ID: mdl-29872765
The shortage of donor islets is a significant obstacle for widespread clinical application of pancreatic islet transplantation. To investigate whether adipose tissue-derived mesenchymal stem cells (ADSCs) induce expansion of transplanted islets, we performed co-transplantation experiments in a mouse model. Streptozotosin (STZ)-induced diabetic mice transplanted with 50 syngeneic islets remained hyperglycemic. However, hyperglycemia was ameliorated gradually when 50 islets were co-transplanted with ADSCs but not separately grafted into the contralateral kidney. Insulin and proinsulin contents of 120-day grafts containing 50 islets co-transplanted with ADSCs were significantly increased compared with those of 50 isolated islets. The Ki67-positive ratios in islets of the naïve pancreas, at 30 and 120 days grafts were 0.23%, 2.12%, and 1.52%, respectively. Ki67-positive cells were predominantly Pdx1+ and insulin+ cells. These results demonstrate that co-transplantation with ADSCs induces proliferation of transplanted islets in mice, suggesting a potential solution for the low efficiency of islet transplantation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Heliyon Año: 2018 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Heliyon Año: 2018 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido