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Histopathological, immunohistochemical, and biomechanical effects of splenectomy on Achilles tendon healing in rats.
Yuksel, Serdar; Guleç, M Akif; Gultekin, Zeki; Caglar, Aysel; Beytemur, Ozan; Alagoz, Ender; Eker, A Akdogan; Subasi, Cansu; Karaoz, Erdal.
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  • Yuksel S; a Bagcilar Training and Research Hospital , Department of Orthopedics and Traumatology , Istanbul , Turkey.
  • Guleç MA; a Bagcilar Training and Research Hospital , Department of Orthopedics and Traumatology , Istanbul , Turkey.
  • Gultekin Z; a Bagcilar Training and Research Hospital , Department of Orthopedics and Traumatology , Istanbul , Turkey.
  • Caglar A; b Bagcilar Training and Research Hospital , Department of Pathology , Istanbul , Turkey.
  • Beytemur O; a Bagcilar Training and Research Hospital , Department of Orthopedics and Traumatology , Istanbul , Turkey.
  • Alagoz E; a Bagcilar Training and Research Hospital , Department of Orthopedics and Traumatology , Istanbul , Turkey.
  • Eker AA; c Yildiz Technical University , Department of Mechanical Engineering , Istanbul , Turkey.
  • Subasi C; d Center for Regenerative Medicine and Stem Cell Research & Manufacturing (LivMedCell) , Istanbul , Turkey.
  • Karaoz E; d Center for Regenerative Medicine and Stem Cell Research & Manufacturing (LivMedCell) , Istanbul , Turkey.
Connect Tissue Res ; 60(2): 200-208, 2019 03.
Article en En | MEDLINE | ID: mdl-29860899
PURPOSE: This study aimed to assess Achilles tendon repair in rats following splenectomy to simulate patients with musculoskeletal system injury who had splenectomy after spleen injury, a situation often seen in orthopedics and traumatology practice. MATERIALS AND METHODS: The study included 32 male Sprague-Dawley rats (10 months old; average weight, 394.5 ± 28.3 g). The rats were fed with standard rodent food ad libitum at 22°C in a dark environment for 12 h. They were divided into two groups, namely the splenectomy (total splenectomy and Achilles tendon repair) and control groups (only Achilles tendon repair; n = 16). Four weeks after the surgery, the rats were euthanized, and their Achilles tendons were examined histopathologically, immunohistochemically, and biomechanically. RESULTS: In the splenectomy group, proinflammatory cytokines, such as interleukin-1ß, tumor necrosis factor-α, and interferon-γ, showed significantly lower values than those in the control group (p ˂0.01); moreover, the levels of anti-inflammatory cytokines like vascular endothelial growth factor, transforming growth factor-ß1, interleukin-2, interleukin-10, and hepatocyte growth factor were significantly higher than in the control group (p ˂ 0.001). The average ultimate tensile strengths were 2.58 ± 0.5 in the splenectomy and 2.78 ± 0.3 in the control group (p = 0.043). The average εUTS values were 0.33 ± 0.5 in the splenectomy and 0.44 ± 0.1 in the control group (p = 0.021). CONCLUSION: Splenectomy may positively influence Achilles tendon healing through modification of the proinflammatory/anti-inflammatory ratio in favor of anti-inflammatory cytokines by causing a decrease in spleen-originated inflammatory cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tendón Calcáneo / Esplenectomía / Cicatrización de Heridas Límite: Animals Idioma: En Revista: Connect Tissue Res Año: 2019 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tendón Calcáneo / Esplenectomía / Cicatrización de Heridas Límite: Animals Idioma: En Revista: Connect Tissue Res Año: 2019 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Reino Unido