Your browser doesn't support javascript.
loading
Acute exposure to zinc oxide nanoparticles critically disrupts operation of the respiratory neural network in neonatal rat.
Nicolosi, Angelo; Cardoit, Laura; Pasquereau, Pierrick; Jaillet, Christèle; Thoby-Brisson, Muriel; Juvin, Laurent; Morin, Didier.
Afiliación
  • Nicolosi A; University of Bordeaux, CNRS, UMR 5287, INCIA, 33000 Bordeaux, France.
  • Cardoit L; University of Bordeaux, CNRS, UMR 5287, INCIA, 33000 Bordeaux, France.
  • Pasquereau P; Bordeaux Institute of Technology, Department of Health, Safety & Environment, 33170 Gradignan, France.
  • Jaillet C; University of Bordeaux, Centre de Recherche Paul Pascal, 33600 Pessac, France; Bordeaux Institute of Technology, Department of Health, Safety & Environment, 33170 Gradignan, France.
  • Thoby-Brisson M; University of Bordeaux, CNRS, UMR 5287, INCIA, 33000 Bordeaux, France.
  • Juvin L; University of Bordeaux, CNRS, UMR 5287, INCIA, 33000 Bordeaux, France.
  • Morin D; University of Bordeaux, CNRS, UMR 5287, INCIA, 33000 Bordeaux, France; Bordeaux Institute of Technology, Department of Health, Safety & Environment, 33170 Gradignan, France. Electronic address: didier.morin@u-bordeaux.fr.
Neurotoxicology ; 67: 150-160, 2018 07.
Article en En | MEDLINE | ID: mdl-29860053
Due to their extremely small size that gives them unique physicochemical properties, nanoparticles (NPs) are used in the production of everyday materials. However, NPs can accumulate in body organs and could cause various diseases. Moreover, NPs that cross biological membranes such as the blood-brain barrier can aggregate in the brain and potentially produce neuronal damage. Although studies have reported the effects of diverse NPs on the bioelectrical properties of individual neurons, their potential influences on the operation of whole neuronal networks have not been documented. Here, we aimed to evaluate the effects of an acute exposure to zinc oxide (ZnO) NPs on the central neural networks responsible for mammalian respiratory rhythm generation. Using an isolated ex vivo brainstem-spinal cord preparation from neonatal rat in which the circuitry for the central respiratory command remained intact, we show that ZnO NPs accelerate, then profoundly disrupt respiratory-related activity produced by the pre-Bötzinger complex (preBötC) responsible for inspiratory rhythm generation. Consequently, a sudden and definitive cessation of respiratory-related activity occurs in ZnO NPs-exposed preparations. Part of these effects is related to zinc ions released from NPs. Using brainstem slice preparations containing the preBötC network, whole-cell patch-clamp recordings revealed that ZnO NPs depolarize preBötC inspiratory neurons and affect their bioelectrical properties by reducing the amplitude of action potentials, thereby leading to a depression of intra-network activity and the ultimate termination of respiratory rhythmogenesis. These findings support the conclusion that ZnO NPs may have deleterious effects on the central respiratory centers of newborn mammals.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Centro Respiratorio / Protectores Solares / Óxido de Zinc / Nanopartículas / Red Nerviosa Límite: Animals Idioma: En Revista: Neurotoxicology Año: 2018 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Centro Respiratorio / Protectores Solares / Óxido de Zinc / Nanopartículas / Red Nerviosa Límite: Animals Idioma: En Revista: Neurotoxicology Año: 2018 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Países Bajos