Redox Equivalents and Mitochondrial Bioenergetics.

Roede, James R; Go, Young-Mi; Jones, Dean P

Roede, James R; Go, Young-Mi; Jones, Dean P.

Afiliación

Roede JR; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado, Aurora, CO, USA. james.roede@ucdenver.edu.
Go YM; Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Emory University, Atlanta, GA, USA.
Jones DP; Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Emory University, Atlanta, GA, USA.

Methods Mol Biol ; 1782: 197-227, 2018.

Article en En | MEDLINE | ID: mdl-29851002

RESUMEN

Mitochondrial energy metabolism depends upon high-flux and low-flux electron transfer pathways. The former provide the energy to support chemiosmotic coupling for oxidative phosphorylation. The latter provide mechanisms for signaling and control of mitochondrial functions. Few practical methods are available to measure rates of individual mitochondrial electron transfer reactions; however, a number of approaches are available to measure steady-state redox potentials (E h) of donor/acceptor couples, and these can be used to gain insight into rate controlling reactions as well as mitochondrial bioenergetics. Redox changes within the respiratory electron transfer pathway are quantified by optical spectroscopy and measurement of changes in autofluorescence. Low-flux pathways involving thiol/disulfide redox couples are measured by redox Western blot and mass spectrometry-based redox proteomics. Together, the approaches provide the opportunity to develop integrated systems biology descriptions of mitochondrial redox signaling and control mechanisms.

Asunto(s)

Metabolismo Energético; Espectrometría de Masas/métodos; Mitocondrias/metabolismo; Proteómica/métodos; Western Blotting/instrumentación; Western Blotting/métodos; Técnicas de Cultivo de Célula/instrumentación; Técnicas de Cultivo de Célula/métodos; Línea Celular Tumoral; Cromatografía Líquida de Alta Presión/instrumentación; Cromatografía Líquida de Alta Presión/métodos; Cisteína/metabolismo; Transporte de Electrón; Glutatión/metabolismo; Células HT29; Humanos; Espectrometría de Masas/instrumentación; Oxidación-Reducción; Proteómica/instrumentación; S-Nitrosotioles/metabolismo

Palabras clave

Cytochromes; Glutathione; Hydrogen peroxide; NADH; NADH dehydrogenase; NADPH; Peroxiredoxin; Redox Western blot; Redox proteomics; Thioredoxin-2; Ubiquinone

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas / Proteómica / Metabolismo Energético / Mitocondrias Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google