Longevity-Related Gene Transcriptomic Signature in Glioblastoma Multiforme.

Fawzy, Manal S; Badran, Dahlia I; Al Ageeli, Essam; Al-Qahtani, Saeed Awad M; Alghamdi, Saleh Ali; Helal, Ghada M; Toraih, Eman A

Fawzy, Manal S; Badran, Dahlia I; Al Ageeli, Essam; Al-Qahtani, Saeed Awad M; Alghamdi, Saleh Ali; Helal, Ghada M; Toraih, Eman A.

Afiliación

Fawzy MS; Department of Medical Biochemistry, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt.
Badran DI; Department of Medical Biochemistry, Faculty of Medicine, Northern Border University, Arar, Saudi Arabia.
Al Ageeli E; Department of Medical Biochemistry, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt.
Al-Qahtani SAM; Center of excellence in Molecular and Cellular Medicine, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Alghamdi SA; Department of Clinical Biochemistry (Medical Genetics), Faculty of Medicine, Jazan University, Jazan, Saudi Arabia.
Helal GM; Department of Physiology, Faculty of Medicine, Taibah University, Almadinah Almunawwarah, Saudi Arabia.
Toraih EA; Medical Genetics, Clinical Laboratory Department, College of Applied Medical Sciences, Taif University, Taif, Saudi Arabia.

Oxid Med Cell Longev ; 2018: 8753063, 2018.

Article en En | MEDLINE | ID: mdl-29849920

RESUMEN

Glioblastoma multiforme (GBM) (grade IV astrocytoma) has been assumed to be the most fatal type of glioma with low survival and high recurrence rates, even after prompt surgical removal and aggressive courses of treatment. Transcriptional reprogramming to stem cell-like state could explain some of the deregulated molecular signatures in GBM disease. The present study aimed to quantify the expression profiling of longevity-related transcriptional factors SOX2, OCT3/4, and NANOG to evaluate their diagnostic and performance values in high-grade gliomas. Forty-four specimens were obtained from glioblastoma patients (10 females and 34 males). Quantitative real-time polymerase chain reaction was applied for relative gene expression quantification. In silico network analysis was executed. NANOG and OCT3/4 mRNA expression levels were significantly downregulated while that of SOX2 was upregulated in cancer compared to noncancer tissues. Receiver operating characteristic curve analysis showed high diagnostic performance of NANOG and OCT3/4 than SOX2. However, the aberrant expressions of the genes studied were not associated with the prognostic variables in the current population. In conclusion, the current study highlighted the aberrant expression of certain longevity-associated transcription factors in glioblastoma multiforme which may direct the attention towards new strategies in the treatment of such lethal disease.

Asunto(s)

Neoplasias Encefálicas/genética; Glioblastoma/genética; Longevidad/genética; Transcriptoma; Adulto; Área Bajo la Curva; Neoplasias Encefálicas/mortalidad; Neoplasias Encefálicas/patología; Supervivencia sin Enfermedad; Regulación hacia Abajo; Femenino; Glioblastoma/mortalidad; Glioblastoma/patología; Humanos; Estimación de Kaplan-Meier; Modelos Lineales; Masculino; Persona de Mediana Edad; Proteína Homeótica Nanog/genética; Proteína Homeótica Nanog/metabolismo; Estadificación de Neoplasias; Factor 3 de Transcripción de Unión a Octámeros/genética; Factor 3 de Transcripción de Unión a Octámeros/metabolismo; Curva ROC; Factores de Transcripción SOXB1/genética; Factores de Transcripción SOXB1/metabolismo; Regulación hacia Arriba

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Encefálicas / Glioblastoma / Transcriptoma / Longevidad Tipo de estudio: Prognostic_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Oxid Med Cell Longev Asunto de la revista: METABOLISMO Año: 2018 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Estados Unidos

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