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Some coagulase negative Staphylococcus spp. isolated from buffalo can be misidentified as Staphylococcus aureus by phenotypic and Sa442 PCR methods.
de Almeida, Camila C; Pizauro, Lucas J L; Soltes, Glenn A; Slavic, Durda; de Ávila, Fernando A; Pizauro, João M; MacInnes, Janet I.
Afiliación
  • de Almeida CC; Agriculture and Livestock Microbiology Graduation Program, Department of Veterinary Pathology, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, Brazil.
  • Pizauro LJL; Department of Pathobiology, University of Guelph, 50 Stone Rd. East, Guelph, ON, N1G 2W1, Canada.
  • Soltes GA; Department of Veterinary Preventive Medicine and Animal Reproduction, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, Brazil.
  • Slavic D; Department of Pathobiology, University of Guelph, 50 Stone Rd. East, Guelph, ON, N1G 2W1, Canada.
  • de Ávila FA; Department of Pathobiology, University of Guelph, 50 Stone Rd. East, Guelph, ON, N1G 2W1, Canada.
  • Pizauro JM; Animal Health Laboratory, University of Guelph, Post Office 3612, Guelph, ON, N1H 6R8, Canada.
  • MacInnes JI; Department of Veterinary Preventive Medicine and Animal Reproduction, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, Brazil.
BMC Res Notes ; 11(1): 346, 2018 May 30.
Article en En | MEDLINE | ID: mdl-29848377
OBJECTIVE: Staphylococcus aureus is a commonly reported cause of buffalo mastitis. However, its prevalence may be overestimated. The aim of this study was to compare S. aureus identification by conventional phenotypic and genotypic assays versus Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) and novel real-time quantitative PCR tests for the cytochrome oxidase subunit D II (cydB) and staphylocoagulase (coa) genes. RESULTS: From 408 samples obtained from buffalo milk/milking environment, 32 putative S. aureus strains were identified based on characteristic growth on Baird Parker agar, positive catalase reaction, ability to clot rabbit plasma, and positive Sa442 PCR assay. However, in further testing, only 10 of these strains were positive in latex agglutination tests and by MALDI-TOF MS, only eight of the 32 strains were S. aureus while the rest were S. chromogenes (19), S. agnetis (3), S. cohnii (1), or S. xylosus (1). All eight strains identified as S. aureus by MALDI-TOF analysis and confirmed by 16S RNA gene sequencing were positive in a S. aureus-specific cydB PCR test. As well, 7/8 S. aureus strains were PCR positive in a real-time coa PCR test as were 2/69 S. chromogenes and the lone S. xylosus strain tested.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Staphylococcus / Staphylococcus aureus / Análisis de Secuencia de ARN / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Reacción en Cadena en Tiempo Real de la Polimerasa / Mastitis Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: BMC Res Notes Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Staphylococcus / Staphylococcus aureus / Análisis de Secuencia de ARN / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Reacción en Cadena en Tiempo Real de la Polimerasa / Mastitis Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: BMC Res Notes Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido