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Investigation of dose-rate effects and cell-cycle distribution under protracted exposure to ionizing radiation for various dose-rates.
Matsuya, Yusuke; McMahon, Stephen J; Tsutsumi, Kaori; Sasaki, Kohei; Okuyama, Go; Yoshii, Yuji; Mori, Ryosuke; Oikawa, Joma; Prise, Kevin M; Date, Hiroyuki.
Afiliación
  • Matsuya Y; Graduate School of Health Sciences, Hokkaido University, Sapporo, 060-0812, Japan.
  • McMahon SJ; Centre for Cancer Research & Cell Biology, Queen's University Belfast, Belfast, BT9 7AE, UK.
  • Tsutsumi K; Faculty of Health Sciences, Hokkaido University, Sapporo, 060-0812, Japan.
  • Sasaki K; Faculty of Health Sciences, Hokkaido University of Science, Sapporo, 006-8585, Japan.
  • Okuyama G; Faculty of Health Sciences, Hokkaido University of Science, Sapporo, 006-8585, Japan.
  • Yoshii Y; Biological Research, Education and Instrumentation Center, Sapporo Medical University, Sapporo, 060-8556, Japan.
  • Mori R; Graduate School of Health Sciences, Hokkaido University, Sapporo, 060-0812, Japan.
  • Oikawa J; Graduate School of Health Sciences, Hokkaido University, Sapporo, 060-0812, Japan.
  • Prise KM; Centre for Cancer Research & Cell Biology, Queen's University Belfast, Belfast, BT9 7AE, UK.
  • Date H; Faculty of Health Sciences, Hokkaido University, Sapporo, 060-0812, Japan. date@hs.hokudai.ac.jp.
Sci Rep ; 8(1): 8287, 2018 05 29.
Article en En | MEDLINE | ID: mdl-29844494
During exposure to ionizing radiation, sub-lethal damage repair (SLDR) competes with DNA damage induction in cultured cells. By virtue of SLDR, cell survival increases with decrease of dose-rate, so-called dose-rate effects (DREs). Here, we focused on a wide dose-rate range and investigated the change of cell-cycle distribution during X-ray protracted exposure and dose-response curves via hybrid analysis with a combination of in vitro experiments and mathematical modelling. In the course of flow-cytometric cell-cycle analysis and clonogenic assays, we found the following responses in CHO-K1 cells: (1) The fraction of cells in S phase gradually increases during 6 h exposure at 3.0 Gy/h, which leads to radio-resistance. (2) Slight cell accumulation in S and G2/M phases is observed after exposure at 6.0 Gy/h for more than 10 hours. This suggests that an increase of SLDR rate for cells in S phase during irradiation may be a reproducible factor to describe changes in the dose-response curve at dose-rates of 3.0 and 6.0 Gy/h. By re-evaluating cell survival for various dose-rates of 0.186-60.0 Gy/h considering experimental-based DNA content and SLDR, it is suggested that the change of S phase fraction during irradiation modulates the dose-response curve and is possibly responsible for some inverse DREs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ciclo Celular / Supervivencia Celular / Relación Dosis-Respuesta en la Radiación Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ciclo Celular / Supervivencia Celular / Relación Dosis-Respuesta en la Radiación Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido