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Molecular basis of growth hormone daily mRNA and protein synthesis in rats.
de Castro Barbosa, T; Salgueiro, R B; Serrano-Nascimento, C; Amaral, F G; Cipolla-Neto, J; Nunes, M T.
Afiliación
  • de Castro Barbosa T; Karolinska Institutet, Department of Medicine H7, Huddinge, Sweden; University of Sao Paulo, Department of Physiology and Biophysics, Sao Paulo, Brazil. Electronic address: thais.de-castro-barbosa@ki.se.
  • Salgueiro RB; University of Sao Paulo, Department of Physiology and Biophysics, Sao Paulo, Brazil.
  • Serrano-Nascimento C; University of Sao Paulo, Department of Physiology and Biophysics, Sao Paulo, Brazil; Hospital Israelita Albert Einstein, Sao Paulo, Brazil.
  • Amaral FG; University of Sao Paulo, Department of Physiology and Biophysics, Sao Paulo, Brazil; Federal University of Sao Paulo, Department of Physiology, Sao Paulo, Brazil.
  • Cipolla-Neto J; University of Sao Paulo, Department of Physiology and Biophysics, Sao Paulo, Brazil.
  • Nunes MT; University of Sao Paulo, Department of Physiology and Biophysics, Sao Paulo, Brazil.
Life Sci ; 207: 36-41, 2018 Aug 15.
Article en En | MEDLINE | ID: mdl-29842863
AIMS: Daily and seasonal rhythms coordinate the endocrine and metabolic functions. The pituitary gland is the master regulator of several endocrine activities, and its function is classically regulated by endocrine signals from its target glands as well as from the hypothalamus. The growth hormone (GH) produced and secreted by the anterior pituitary presents a pulsatile secretion throughout the 24-hour cycle. However, the molecular mechanisms regulating the daily pattern of GH secretion are still unclear. Herein we investigated whether circadian GH mRNA and protein synthesis is modulated by acute adjustments in the stability and expression of GH mRNA. MAIN METHODS: GH mRNA and protein content were evaluated by real-time PCR and Western blotting, respectively, in pituitary gland of rats euthanized every 3 h during a 24-h period at the Zeitgeber times (ZT3 to ZT24). The GH mRNA poly(A) tail length was determined by RACE-PAT assay. KEY FINDINGS: We identified two main peaks of GH mRNA level in the pituitary gland of rats; one in the middle of the light-cycle and another in the middle of the dark-cycle. The latter was associated with an increase in pituitary GH protein content. Interestingly, an increment in the poly(A) tail length of the GH transcript was observed in association to reduced migration rate of the GH transcript and increased mRNA content in the dark-cycle period. SIGNIFICANCE: Our findings provide evidence that changes in the GH mRNA poly(A) length may underlie the circadian pattern of GH mRNA and protein levels in the pituitary gland of rats.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hipófisis / ARN Mensajero / Hormona del Crecimiento / Ritmo Circadiano Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Life Sci Año: 2018 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hipófisis / ARN Mensajero / Hormona del Crecimiento / Ritmo Circadiano Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Life Sci Año: 2018 Tipo del documento: Article Pais de publicación: Países Bajos