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Cellular mechanisms to survive salt in the halophyte Cakile maritima.
Arbelet-Bonnin, Delphine; Ben Hamed-Laouti, Ibtissem; Laurenti, Patrick; Abdelly, Chedly; Ben Hamed, Karim; Bouteau, François.
Afiliación
  • Arbelet-Bonnin D; Université Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain, Paris, France.
  • Ben Hamed-Laouti I; Université Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain, Paris, France; Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie de Borj Cedria, University of Carthage-Tunis, BP 901, 2050 Hammam Lif, Tunisia.
  • Laurenti P; Université Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain, Paris, France.
  • Abdelly C; Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie de Borj Cedria, University of Carthage-Tunis, BP 901, 2050 Hammam Lif, Tunisia.
  • Ben Hamed K; Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie de Borj Cedria, University of Carthage-Tunis, BP 901, 2050 Hammam Lif, Tunisia.
  • Bouteau F; Université Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain, Paris, France. Electronic address: francois.bouteau@univ-paris-diderot.fr.
Plant Sci ; 272: 173-178, 2018 Jul.
Article en En | MEDLINE | ID: mdl-29807589
We recently identified two behaviours in cultured cells of the salt accumulating halophyte Cakile maritima: one related to a sustained depolarization due to Na+ influx through the non-selective cation channels leading to programmed cell death of these cells, a second one related to a transient depolarization allowing cells to survive (Ben Hamed-Laouti, 2016). In this study, we considered at the cellular level mechanisms that could participate to the exclusion of Na+ out of the cell and thus participate in the regulation of the internal contents of Na+ and cell survival. Upon addition of NaCl in the culture medium of suspension cells of C. maritima, we observed a rapid influx of Na+ followed by an efflux dependent of the activity of plasma membrane H+-ATPases, in accordance with the functioning of a Na+/H+ antiporter and the ability of some cells to repolarize. The Na+ efflux was shown to be dependent on Na+-dependent on Ca2+ influx like the SOS1 Na+/H+ antiporter. We further could observe in response to salt addition, an early production of singlet oxygen (1O2) probably due to peroxidase activities. This early 1O2 production seemed to be a prerequisite to the Na+ efflux. Our findings suggest that in addition to the pathway leading to PCD (Ben Hamed-Laouti, 2016), a second pathway comprising an SOS-like system could participate to the survival of a part of the C. maritima cultured cells challenged by salt stress.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Brassicaceae / Plantas Tolerantes a la Sal Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Sci Año: 2018 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Irlanda
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Brassicaceae / Plantas Tolerantes a la Sal Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Sci Año: 2018 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Irlanda