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Candidate Biomarkers for Oral Squamous Cell Carcinoma: Differential Expression of Oxidative Stress-Related Genes
Pedro, Nayara Fernandes; Biselli, Joice Matos; Maniglia, José Victor; Santi-Neto, Dalísio de; Pavarino, Érika Cristina; Goloni-Bertollo, Eny Maria; Biselli-Chicote, Patrícia Matos.
Afiliación
  • Pedro NF; Genetics and Molecular Biology Research Unit (UPGEM), São José do Rio Preto Medical School (FAMERP), Avenida Brigadeiro Faria Lima, 5416, 15090-000, São Pedro, São José do Rio Preto, São Paulo, Brazil. Email: patriciabiselli@famerp.br
Asian Pac J Cancer Prev ; 19(5): 1343-1349, 2018 May 26.
Article en En | MEDLINE | ID: mdl-29802697
Background: Alteration in the biotransformation of exogenous compounds can result in production of reactive oxygen species (ROS), which can predispose cells to malignant transformation in the head and neck. This study aimed to evaluate the expression of genes involved in antioxidant metabolism in the oral squamous cell carcinoma (OSCC). Methods: The expression of eighty-four genes was evaluated in OSCC and non-tumor tissues by quantitative real-time polymerase chain reaction using the TaqMan Gene Expression Array. The biological mechanisms related to the differentially expressed genes were investigated using Gene ­ NCBI, KEGG, UNIPROT and REACTOME databases. Results: Twenty-one genes encoding enzymes involved in antioxidant metabolism were differentially expressed in the OSCC case. Four genes (ATOX1, PRDX4, PRNP, and SOD2) were up-regulated, and seventeen (ALOX12, CAT, CSDE1, DHCR24, DUOX1, DUOX2, EPHX2, GLRX2, GPX3, GSR, GSTZ1, MGST3, PRDX1, OXR1, OXSR1, SOD1, and SOD3) were down-regulated. We identified 14 possible novel biomarkers for OSCC. The differentially expressed genes appeared related to important biological processes involved in carcinogenesis, such as inflammation, angiogenesis, apoptosis, genomic instability, invasion, survival, and cell proliferation. Conclusions: Our study identified novel biomarkers which might warrant further investigation regarding OSCC pathogenesis since the altered expression in the genes can modulate biological processes related to oxidative stress and predispose cells to malignant transformation in the oral cavity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Boca / Carcinoma de Células Escamosas / Biomarcadores de Tumor / Regulación Neoplásica de la Expresión Génica / Estrés Oxidativo / Perfilación de la Expresión Génica Tipo de estudio: Observational_studies / Prognostic_studies Límite: Aged / Female / Humans / Male Idioma: En Revista: Asian Pac J Cancer Prev Asunto de la revista: NEOPLASIAS Año: 2018 Tipo del documento: Article Pais de publicación: Tailandia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Boca / Carcinoma de Células Escamosas / Biomarcadores de Tumor / Regulación Neoplásica de la Expresión Génica / Estrés Oxidativo / Perfilación de la Expresión Génica Tipo de estudio: Observational_studies / Prognostic_studies Límite: Aged / Female / Humans / Male Idioma: En Revista: Asian Pac J Cancer Prev Asunto de la revista: NEOPLASIAS Año: 2018 Tipo del documento: Article Pais de publicación: Tailandia