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Carnosic acid impedes cell growth and enhances anticancer effects of carmustine and lomustine in melanoma.
Lin, Kun-I; Lin, Chih-Chien; Kuo, Shyh-Ming; Lai, Jui-Chi; Wang, You-Qi; You, Huey-Ling; Hsu, Mei-Ling; Chen, Chang-Han; Shiu, Li-Yen.
Afiliación
  • Lin KI; Department of Cosmetic Science, Providence University, Taichung, Taiwan, R.O.C.
  • Lin CC; Departments of Obstetrics and Gynecology, Chang Bing Show Chwan Memorial Hospital, Lukang Zhen, Changhua County, Taiwan, R.O.C.
  • Kuo SM; Department of Cosmetic Science, Providence University, Taichung, Taiwan, R.O.C.
  • Lai JC; Department of Biomedical Engineering, I-Shou University, Taiwan, R.O.C.
  • Wang YQ; Department of Biological Science and Technology, I-SHOU University, Kaohsiung, Taiwan, R.O.C.
  • You HL; Department of Biological Science and Technology, I-SHOU University, Kaohsiung, Taiwan, R.O.C.
  • Hsu ML; Department of Laboratory Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan, R.O.C.
  • Chen CH; Department of Medical Laboratory Sciences and Biotechnology, Fooyin University, Kaohsiung, Taiwan, R.O.C.
  • Shiu LY; Department of Laboratory Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan, R.O.C.
Biosci Rep ; 38(4)2018 08 31.
Article en En | MEDLINE | ID: mdl-29789400
Carnosic acid (CA), a major polyphenolic diterpene present in Rosmarinus officinalis, has been reported to have multiple functions, including antitumor activity. The MTT assay, BrdU incorporation, wound healing, and colony formation were used to detect melanoma B16F10 cell growth and proliferation. Flow cytometry was used for cell cycle detection. p21 and p27 expression was detected by Western blotting. B16F10 cell xenograft model was established, and treated with CA, carmustine (BCNU), or lomustine (CCNU). The present study found that CA exhibits significant growth inhibition and cell cycle arrest in melanoma B16F10 cells. We also found that CA triggers cell cycle arrest at G0/G1 phase, and enhances p21 expression. Additionally, CA can enhance BCNU- and CCNU-mediated cytotoxicity and cell cycle arrest in B16F10 cells. Finally, we found that CA inhibits tumor growth, and reduces the values of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in vivo The present study study concluded that CA may be safe and useful as a novel chemotherapeutic agent.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Melanoma Experimental / Carmustina / Antineoplásicos Alquilantes / Abietanos / Lomustina / Antioxidantes Límite: Animals Idioma: En Revista: Biosci Rep Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Melanoma Experimental / Carmustina / Antineoplásicos Alquilantes / Abietanos / Lomustina / Antioxidantes Límite: Animals Idioma: En Revista: Biosci Rep Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido